Literature summary for 2.3.1.194 extracted from

McQualter, R.B.; Petrasovits, L.A.; Gebbie, L.K.; Schweitzer, D.; Blackman, D.M.; Chrysanthopoulos, P.; Hodson, M.P.; Plan, M.R.; Riches, J.D.; Snell, K.D.; Brumbley, S.M.; Nielsen, L.K.
The use of an acetoacetyl-CoA synthase in place of a beta-ketothiolase enhances poly-3-hydroxybutyrate production in sugarcane mesophyll cells (2015), Plant Biotechnol. J., 13, 700-707 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene nphT7, functional recombinant expression in sugarcane (Saccharum sp.) cultivar Q117, coexpression of gene nphT7 with genes phaB and phaC	Streptomyces sp. CL190

Protein Variants

Protein Variants	Comment	Organism
additional information	engineering the production of polyhydroxyalkanoates (PHAs) into high biomass bioenergy crops has the potential to provide a sustainable supply of bioplastics and energy from a single plant feedstock. One of the major challenges in engineering C4 plants for the production of poly[(R)-3-hydroxybutyrate] (PHB) is the significantly lower level of polymer produced in the chloroplasts of mesophyll cells compared to bundle sheath cells, thereby limiting the full PHB yield potential of the plant. The access to substrate for PHB synthesis may limit polymer production in mesophyll cell chloroplasts. The use of an acetoacetyl-CoA synthase, that catalyses the conversion of acetyl-CoA and malonyl-CoA to acetoacetyl-CoA with the release of carbon dioxide, in place of a beta-ketothiolase, the first enzyme in the bacterial PHA pathway, enhances poly-3-hydroxybutyrate production in sugarcane mesophyll cells. The engineered cells shows increased production of PHB and increased polymer molecular weight. PhaA is the main contributor to low PHB yield in mesophyll cells. Phenotypes of transgenic sugarcane leafs, overview	Streptomyces sp. CL190

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + malonyl-CoA	Streptomyces sp. CL190	-	acetoacetyl-CoA + CoA + CO2	-	ir

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp. CL190	D7URV0	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + malonyl-CoA	-	Streptomyces sp. CL190	acetoacetyl-CoA + CoA + CO2	-	ir

Synonyms

Synonyms	Comment	Organism
AACS	-	Streptomyces sp. CL190
Acetoacetyl-CoA synthase	-	Streptomyces sp. CL190
NphT7	-	Streptomyces sp. CL190

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Release 2023.1 (January 2023)