BRENDA - Enzyme Database show
show all sequences of 2.3.1.193

The RNA acetyltransferase driven by ATP hydrolysis synthesizes N4-acetylcytidine of tRNA anticodon

Ikeuchi, Y.; Kitahara, K.; Suzuki, T.; EMBO J. 27, 2194-2203 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
hexahistidine-tagged YpfI
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic parameters for ATP-hydrolysis or GTP-hydrolysis
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA
Escherichia coli
tRNAMet cytidine acetyltransferase acetylates the wobble base C34 of the elongation-specific tRNAMet. TmcA specifically recognizes the anticodon stem of tRNAMet, thus distinguishing between tRNAMet and tRNAIle2, which is structurally similar and has the same anticodon loop
[elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA
tRNAMet cytidine acetyltransferase acetylates the wobble base C34 of the elongation-specific tRNAMet. TmcA specifically recognizes the anticodon stem of tRNAMet, thus distinguishing between tRNAMet and tRNAIle2, which is structurally similar and has the same anticodon loop
703441
Escherichia coli
[elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A
-
-
-
?
[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA
ATP/GTP hydrolysis by TmcA is stimulated in the presence of acetyl-CoA and tRNAMet. TmcA hydrolyses ATP or GTP between the beta- and gamma-phosphates, as shown by the detection of labelled ADP (or GDP). A mutation study reveals that Escherichia coli TmcA strictly discriminates elongator tRNAMet from the structurally similar tRNAIle by mainly recognizing the C27–G43 pair in the anticodon stem
703441
Escherichia coli
[elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A
-
-
-
?
[elongator tRNAMet]-cytidine34 + GTP + acetyl-CoA
-
703441
Escherichia coli
[elongator tRNAMet]-N4-acetylcytidine34 + GDP + phosphate + coenzyme A
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic parameters for ATP-hydrolysis or GTP-hydrolysis
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
hexahistidine-tagged YpfI
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic parameters for ATP-hydrolysis or GTP-hydrolysis
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA
Escherichia coli
tRNAMet cytidine acetyltransferase acetylates the wobble base C34 of the elongation-specific tRNAMet. TmcA specifically recognizes the anticodon stem of tRNAMet, thus distinguishing between tRNAMet and tRNAIle2, which is structurally similar and has the same anticodon loop
[elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA
tRNAMet cytidine acetyltransferase acetylates the wobble base C34 of the elongation-specific tRNAMet. TmcA specifically recognizes the anticodon stem of tRNAMet, thus distinguishing between tRNAMet and tRNAIle2, which is structurally similar and has the same anticodon loop
703441
Escherichia coli
[elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A
-
-
-
?
[elongator tRNAMet]-cytidine34 + ATP + acetyl-CoA
ATP/GTP hydrolysis by TmcA is stimulated in the presence of acetyl-CoA and tRNAMet. TmcA hydrolyses ATP or GTP between the beta- and gamma-phosphates, as shown by the detection of labelled ADP (or GDP). A mutation study reveals that Escherichia coli TmcA strictly discriminates elongator tRNAMet from the structurally similar tRNAIle by mainly recognizing the C27–G43 pair in the anticodon stem
703441
Escherichia coli
[elongator tRNAMet]-N4-acetylcytidine34 + ADP + phosphate + coenzyme A
-
-
-
?
[elongator tRNAMet]-cytidine34 + GTP + acetyl-CoA
-
703441
Escherichia coli
[elongator tRNAMet]-N4-acetylcytidine34 + GDP + phosphate + coenzyme A
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic parameters for ATP-hydrolysis or GTP-hydrolysis
Escherichia coli
General Information
General Information
Commentary
Organism
malfunction
a tmcA deletion strain does not show a growth defect. Thus, this modification does not appear to be required for faithful translation in an otherwise wild-type strain. A tmcA/dusC double deletion strain has a severe cold-sensitive growth defect
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
a tmcA deletion strain does not show a growth defect. Thus, this modification does not appear to be required for faithful translation in an otherwise wild-type strain. A tmcA/dusC double deletion strain has a severe cold-sensitive growth defect
Escherichia coli
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic parameters for ATP-hydrolysis or GTP-hydrolysis
Escherichia coli
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic parameters for ATP-hydrolysis or GTP-hydrolysis
Escherichia coli
Other publictions for EC 2.3.1.193
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
703445
Chimnaronk
RNA helicase module in an acet ...
Escherichia coli
EMBO J.
28
1362-1373
2009
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703441
Ikeuchi
The RNA acetyltransferase driv ...
Escherichia coli
EMBO J.
27
2194-2203
2008
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1
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3
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3
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