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Literature summary for 2.3.1.189 extracted from

  • Vetting, M.W.; Roderick, S.L.; Yu, M.; Blanchard, J.S.
    Crystal structure of mycothiol synthase (Rv0819) from Mycobacterium tuberculosis shows structural homology to the GNAT family of N-acetyltransferases (2003), Protein Sci., 12, 1954-1959.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
the Rv0819 gene encoding MshD (residues 1–315) is amplified from Mycobacterium tuberculosis genomic DNA using PCR and cloned into the pET28a(+) plasmid. This vector construction adds six histidine residues and a thrombin cleavage sequence to the N-terminus to facilitate protein purification. Expression in Escherichia coli stain BL21(DE3)pLysS Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion under oil method Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
desacetylmycothiol + acetyl-CoA Mycobacterium tuberculosis
-
 mycothiol + coenzyme-A
-
 ?
desacetylmycothiol + acetyl-CoA Mycobacterium tuberculosis H37Rv
-
 mycothiol + coenzyme-A
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WJM7
-
-
Mycobacterium tuberculosis H37Rv P9WJM7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
desacetylmycothiol + acetyl-CoA
-
 Mycobacterium tuberculosis mycothiol + coenzyme-A
-
 ?
desacetylmycothiol + acetyl-CoA
-
 Mycobacterium tuberculosis H37Rv mycothiol + coenzyme-A
-
 ?