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Literature summary for 2.3.1.184 extracted from

  • Gould, T.A.; Herman, J.; Krank, J.; Murphy, R.C.; Churchill, M.E.
    Specificity of acyl-homoserine lactone synthases examined by mass spectrometry (2006), J. Bacteriol., 188, 773-783.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.184

Cloned(Commentary)

Cloned (Comment) Organism
genes esaI and lasI, expression of wild-type and mutant enzymes in Escherichia coli Pantoea stewartii

Protein Variants

Protein Variants Comment Organism
T140A site-directed mutagenesis of EsaI, the mutant shows altered substrate acyl-chain length specificity compared to the wild-type enzyme Pantoea stewartii
T142A site-directed mutagenesis of LasI, the mutant shows slightly altered substrate acyl-chain length specificity compared to the wild-type enzyme Pantoea stewartii
T142G site-directed mutagenesis of LasI, the mutant shows reduced activity and altered substrate acyl-chain length specificity compared to the wild-type enzyme Pantoea stewartii
T142S site-directed mutagenesis of LasI, the mutant shows slightly altered substrate acyl-chain length specificity compared to the wild-type enzyme Pantoea stewartii
T144V site-directed mutagenesis of LasI, the mutant shows reduced activity and altered substrate acyl-chain length specificity compared to the wild-type enzyme Pantoea stewartii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pantoea stewartii EsaI and LasI produce N-acyl-L-homoserine lactones signaling molecules as autoinducers of quorum-sensing signaling and regulation of gene expression ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pantoea stewartii
-
 genes esaI and lasI
-

Reaction

Reaction Comment Organism Reaction ID
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone Thr140 in the acyl-chain binding pocket directs the enzyme towards production of 3-oxohomoserine lactones and is involved in determination of substrate specificity of EsaI Pantoea stewartii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information EsaI and LasI produce N-acyl-L-homoserine lactones signaling molecules as autoinducers of quorum-sensing signaling and regulation of gene expression Pantoea stewartii ?
-
 ?
additional information substrate specificities of wild-type and mutant EsaI and LasI, overview Pantoea stewartii ?
-
 ?
S-adenosyl-L-methionine + 3-oxodecanoyl-[acyl-carrier protein] substrate of wild-type and mutant LasI, overview Pantoea stewartii 5'-methylthioadenosine + N-3-oxodecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + 3-oxododecanoyl-[acyl-carrier protein] substrate of wild-type and mutant LasI, overview Pantoea stewartii 5'-methylthioadenosine + N-3-oxododecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein] substrate of wild-type EsaI and EsaI mutant T140A Pantoea stewartii 5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + 3-oxotetradecanoyl-[acyl-carrier protein] substrate of wild-type and mutant LasI, overview Pantoea stewartii 5'-methylthioadenosine + N-3-oxotetradecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + 3-oxoundecanoyl-[acyl-carrier protein] substrate of wild-type and mutant LasI, overview Pantoea stewartii 5'-methylthioadenosine + N-3-oxoundecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein] preferred substrate of EsaI mutant T140A, no substrate of wild-type EsaI Pantoea stewartii 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Synonyms

Synonyms Comment Organism
AHL synthase
-
 Pantoea stewartii
EsaI
-
 Pantoea stewartii
LasI
-
 Pantoea stewartii