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Literature summary for 2.3.1.184 extracted from

  • Hanzelka, B.L.; Parsek, M.R.; Val, D.L.; Dunlap, P.V.; Cronan, J.E., Jr.; Greenberg, E.P.
    Acylhomoserine lactone synthase activity of the Vibrio fischeri AinS protein (1999), J. Bacteriol., 181, 5766-5770.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.184

Cloned(Commentary)

Cloned (Comment) Organism
gene ainS, expression of AinS as maltose binding protein-fusion enzyme in Escherichia coli strain XL 1-Blue Aliivibrio fischeri

Inhibitors

Inhibitors Comment Organism Structure
5'-methylthioadenosine product inhibition, 51% inhibition at 0.005 mM, 92% inhibition at 0.06 mM Aliivibrio fischeri
S-Adenosyl-D-homocysteine 50% inhibition at 0.25 mM, 85% inhibition at 2.5 mM Aliivibrio fischeri
S-adenosyl-L-homocysteine 16% inhibition at 0.25 mM, 45% inhibition at 2.5 mM Aliivibrio fischeri
[Acyl-carrier-protein] product inhibition, in presence of 10 mM DTT, 26% inhibition at 0.25 mM, 43% inhibition at 0.5 mM Aliivibrio fischeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Aliivibrio fischeri
0.004
-
 octanoyl-[acyl-carrier protein] pH 8.5, 25°C, recombinant MBP-AinS Aliivibrio fischeri
0.015
-
 octanoyl-CoA pH 8.5, 25°C, recombinant MBP-AinS Aliivibrio fischeri
0.023
-
 S-adenosyl-L-methionine pH 8.5, 25°C, recombinant MBP-AinS, with octanoyl-[acyl-carrier-protein] Aliivibrio fischeri
0.061
-
 S-adenosyl-L-methionine pH 8.5, 25°C, recombinant MBP-AinS, with octanoyl-CoA Aliivibrio fischeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
 x * 87000, recombinant MBP-AinS, SDS-PAGE Aliivibrio fischeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3-hydroxybutyryl-[acyl-carrier protein] Aliivibrio fischeri substrate of LuxM 5'-methylthioadenosine + N-3-hydroxybutyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein] Aliivibrio fischeri substrate of LuxI 5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein] Aliivibrio fischeri substrate of AinS 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Organism

Organism UniProt Comment Textmining
Aliivibrio fischeri
-
 genes ainS, luxM, and luxI
-

Purification (Commentary)

Purification (Comment) Organism
recobinant maltose binding protein-fusion AinS from Escherichia coli by amylose affinity chromatography Aliivibrio fischeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Aliivibrio fischeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of AinS, weak activity with S-adenosyl-L-ethionine, and butyryl-[acyl-carrier-protein], no activity with sodium octanoate Aliivibrio fischeri ?
-
 ?
S-adenosyl-L-methionine + 3-hydroxybutyryl-[acyl-carrier protein] substrate of LuxM Aliivibrio fischeri 5'-methylthioadenosine + N-3-hydroxybutyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + 3-oxohexanoyl-[acyl-carrier protein] substrate of LuxI Aliivibrio fischeri 5'-methylthioadenosine + N-3-oxohexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + decanoyl-[acyl-carrier protein] substrate of recombinant MBP-AinS Aliivibrio fischeri 5'-methylthioadenosine + N-decanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein] substrate of recombinant MBP-AinS Aliivibrio fischeri 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + octanoyl-CoA substrate of recombinant MBP-AinS, lower activity Aliivibrio fischeri 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + CoA
-
 ?
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein] substrate of AinS Aliivibrio fischeri 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein] preferred substrate of native AinS and recombinant MBP-AinS Aliivibrio fischeri 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Subunits

Subunits Comment Organism
? x * 87000, recombinant MBP-AinS, SDS-PAGE Aliivibrio fischeri

Synonyms

Synonyms Comment Organism
acylhomoserine lactone synthase
-
 Aliivibrio fischeri
AinS
-
 Aliivibrio fischeri
AinS protein
-
 Aliivibrio fischeri
LuxI
-
 Aliivibrio fischeri
LuxM
-
 Aliivibrio fischeri
More the enzymes are members of the LuxI family of synthases Aliivibrio fischeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
-
 Aliivibrio fischeri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 42 over 60% of maximal activity at 25°C and 37°C, less than 15% of maximal activity at 15°C and 42°C Aliivibrio fischeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Aliivibrio fischeri

pH Range

pH Minimum pH Maximum Comment Organism
8 10 sharp drop of activity below pH 8.0 and above pH 10.0, inactive at pH 6.0 and pH 11.0 Aliivibrio fischeri