Literature summary for 2.3.1.184 extracted from

Watson, W.T.; Murphy, F.V.I.V.; Gould, T.A.; Jambeck, P.; Val, D.L.; Cronan, J.E., Jr.; Beck von Bodman, S.; Churchill, M.E.A.
Crystallization and rhenium MAD phasing of the acyl-homoserinelactone synthase EsaI (2001), Acta Crystallogr. Sect. D, 57, 1945-1949.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of His-tagged enzyme in Escherichia coli straoin BL21(DE3)	Pantoea stewartii subsp. stewartii

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged enzyme, vapor diffusion, 6 mg/ml protein in 0.1 M MES, pH 6.1, 14% PEG 4000, 6% 2-propanol, 0.03% 2-mercaptoethanol, 10 mM EDTA, and 0.5% NaN3, addition of agarose reduces the formation of a protein skin on the drop surface, X-ray diffraction structure determination and analysis at 1.14-1.19 A resolution, MAD phasing, modeling	Pantoea stewartii subsp. stewartii

Organism

Organism	UniProt	Comment	Textmining
Pantoea stewartii subsp. stewartii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis	Pantoea stewartii subsp. stewartii

Synonyms

Synonyms	Comment	Organism
acyl-homoserinelactone synthase	-	Pantoea stewartii subsp. stewartii
AHL synthase	-	Pantoea stewartii subsp. stewartii
EsaI	-	Pantoea stewartii subsp. stewartii

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Release 2023.1 (January 2023)