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Literature summary for 2.3.1.183 extracted from

  • Vinnemeier, J.; Droege-Laser, W.; Pistorius, E.K.; Broer, I.
    Purification and partial characterization of the Streptomyces viridochromogenes Tue494 phosphinothricin-N-acetyltransferase mediating resistance to the herbicide phosphinothricin in transgenic plants (1995), Z. Naturforsch. C, 50, 796-805.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Streptomyces viridochromogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Streptomyces viridochromogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
2 * 20000, about, recombinant enzyme Streptomyces viridochromogenes
40000
-
recombinant enzyme, gel filtration Streptomyces viridochromogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptomyces viridochromogenes the enzyme confers resistance to the herbicide phosphinothricin ?
-
?
additional information Streptomyces viridochromogenes Tue494 the enzyme confers resistance to the herbicide phosphinothricin ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces viridochromogenes
-
-
-
Streptomyces viridochromogenes Tue494
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, anion exchange, hydrophobic interaction, and hydroxylapatite chromatography, followed by gel filtration, to about 90% purity Streptomyces viridochromogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme confers resistance to the herbicide phosphinothricin Streptomyces viridochromogenes ?
-
?
additional information the enzyme confers resistance to the herbicide phosphinothricin Streptomyces viridochromogenes Tue494 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 20000, about, recombinant enzyme Streptomyces viridochromogenes

Synonyms

Synonyms Comment Organism
Pat
-
Streptomyces viridochromogenes
phosphinothricin-N-acetyltransferase
-
Streptomyces viridochromogenes