Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-((4-bromo-3-[(diethylamino)sulfonyl]benzoyl)amino)benzoic acid | benzoylamino-benzoic acid derivative inhibitor-enzyme interaction analysis, overview | Enterococcus faecalis | |
additional information | indole analogue inhibitor-enzyme interaction analysis, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + a malonyl-[acyl-carrier protein] | Escherichia coli | - |
an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + a malonyl-[acyl-carrier protein] | Enterococcus faecalis | - |
an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + a malonyl-[acyl-carrier protein] | Enterococcus faecalis ATCC 700802 | - |
an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | Q820T1 | gene fabH | - |
Enterococcus faecalis ATCC 700802 | Q820T1 | gene fabH | - |
Escherichia coli | P0A6R0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + a malonyl-[acyl-carrier protein] | - |
Escherichia coli | an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + a malonyl-[acyl-carrier protein] | - |
Enterococcus faecalis | an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + a malonyl-[acyl-carrier protein] | - |
Enterococcus faecalis ATCC 700802 | an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-acyl carrier protein synthase III | - |
Escherichia coli |
beta-ketoacyl-acyl carrier protein synthase III | - |
Enterococcus faecalis |
FabH | - |
Escherichia coli |
FabH | - |
Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Escherichia coli | |
acetyl-CoA | - |
Enterococcus faecalis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0016 | - |
pH and temperature not specified in the publication | Enterococcus faecalis | 2-((4-bromo-3-[(diethylamino)sulfonyl]benzoyl)amino)benzoic acid |
General Information | Comment | Organism |
---|---|---|
evolution | enzymes FabH from Gram-positive and Gram-negative bacteria are known to have distinct preferences for acyl-CoA primers. This is functionally important, as the substrate specificity of the enzyme governs the fatty acid profile of the organism. FabH from Escherichia coli and other Gram-negative organisms are selective for acetyl-CoA and produce straight-chain fatty acids, while the FabH enzymes of Gram-positive bacteria such as bacilli prefer branched-chain primers and produce branched-chain fatty acids | Escherichia coli |
evolution | enzymes FabH from Gram-positive and Gram-negative bacteria are known to have distinct preferences for acyl-CoA primers. This is functionally important, as the substrate specificity of the enzyme governs the fatty acid profile of the organism. FabH from Escherichia coli and other Gram-negative organisms are selective for acetyl-CoA and produce straight-chain fatty acids, while the FabH enzymes of Gram-positive bacteria such as bacilli prefer branched-chain primers and produce branched-chain fatty acids | Enterococcus faecalis |
additional information | molecular dynamics simulations to characterize the conformational space accessible to enzyme homologues under native conditions, using the FabH crystal structure PDB ID 1MZS, with a bound indole analogue inhibitor, to start. Analysis of protein-ligand interactions in the ecFabH co-crystal structure | Escherichia coli |
additional information | molecular dynamics simulations to characterize the conformational space accessible to enzyme homologues under native conditions, using the FabH crystal structure PDB ID 3IL5 to start. Analysis of protein-ligand interactions in the efFabH co-crystal structure | Enterococcus faecalis |
physiological function | beta-ketoacyl-acyl carrier protein synthase III (FabH) is an essential enzyme in the FASII pathway, which catalyzes the first step in the pathway, the condensation of acyl-CoA primers with malonyl-ACP | Escherichia coli |
physiological function | beta-ketoacyl-acyl carrier protein synthase III (FabH) is an essential enzyme in the FASII pathway, which catalyzes the first step in the pathway, the condensation of acyl-CoA primers with malonyl-ACP | Enterococcus faecalis |