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Literature summary for 2.3.1.180 extracted from

  • Choi, K.H.; Kremer, L.; Besra, G.S.; Rock, C.O.
    Identification and substrate specificity of beta-ketoacyl (acyl carrier protein) synthase III (mtFabH) from Mycobacterium tuberculosis (2000), J. Biol. Chem., 275, 28201-28207.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene fabH, overexpression of His-tagged enzyme in Mycobacterium bovis strain BCG Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
ampicillin inhibition of the recombinant enzyme in transformed Mycobacterium bovis strain BCG in vivo Mycobacterium tuberculosis
Ethionamide inhibition of the recombinant enzyme in transformed Mycobacterium bovis strain BCG in vivo Mycobacterium tuberculosis
isoniazid inhibition of the recombinant enzyme in transformed Mycobacterium bovis strain BCG in vivo Mycobacterium tuberculosis
isoxyl inhibition of the recombinant enzyme in transformed Mycobacterium bovis strain BCG in vivo Mycobacterium tuberculosis
additional information the mitochondrial enzyme is not affected by cerulenin Mycobacterium tuberculosis
rifampicin inhibition of the recombinant enzyme in transformed Mycobacterium bovis strain BCG in vivo Mycobacterium tuberculosis
thiolactomycin inhibition of the purified enzyme in vitro, and of the recombinant enzyme in transformed Mycobacterium bovis strain BCG in vivo Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + malonyl-[acyl-carrier protein] Mycobacterium tuberculosis the condensation reaction is the first step in elongation in fatty acid synthesis by fatty acid synthase complex acetoacyl-[acyl-carrier protein] + CoA + CO2
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Mycobacterium bovis strain BCG Mycobacterium tuberculosis

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 active site structure a catayltic triad of Cys-His-Asn residues Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
substrate specificity of the purified His-tagged enzyme Mycobacterium tuberculosis
0.00013
-
purified His-tagged enzyme, substrate lauroyl-CoA Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + malonyl-[acyl-carrier protein] the condensation reaction is the first step in elongation in fatty acid synthesis by fatty acid synthase complex Mycobacterium tuberculosis acetoacyl-[acyl-carrier protein] + CoA + CO2
-
?
arachidoyl-CoA + malonyl-[acyl-carrier protein] low activity Mycobacterium tuberculosis 3-oxo-docosanoyl-[acyl-carrier protein] + CoA + CO2
-
?
decanoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis 3-oxo-lauroyl-[acyl-carrier protein] + CoA + CO2
-
?
lauroyl-CoA + malonyl-[acyl-carrier protein] lauroyl-CoA is the preferred primer Mycobacterium tuberculosis 3-oxo-myristoyl-[acyl-carrier protein] + CoA + CO2
-
?
additional information substrate specificity, no activity with isovaleryl-CoA, butyryl-CoA, and acetyl-CoA, the enzyme prefers long chain acyl-CoAs as primers rather than acyl-ACPs Mycobacterium tuberculosis ?
-
?
myristoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis 3-oxo-palmitoyl-[acyl-carrier protein] + CoA + CO2
-
?
octanoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis 3-oxo-decanoyl-[acyl-carrier protein] + CoA + CO2
-
?
palmitoyl-CoA + malonyl-[acyl-carrier protein]
-
Mycobacterium tuberculosis 3-oxo-stearoyl-[acyl-carrier protein] + CoA + CO2
-
?
stearoyl-CoA + malonyl-[acyl-carrier protein] low activity Mycobacterium tuberculosis 3-oxo-arachidoyl-[acyl-carrier protein] + CoA + CO2
-
?

Subunits

Subunits Comment Organism
? x * 43000, recombinant His-tagged enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
beta-ketoacyl (acyl carrier protein) synthase III
-
Mycobacterium tuberculosis
beta-ketoacyl-ACP III
-
Mycobacterium tuberculosis
FabH
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Mycobacterium tuberculosis