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Literature summary for 2.3.1.180 extracted from
- Isolation and characterization of the beta-ketoacyl-acyl carrier protein synthase III gene (fabH) from Escherichia coli K-12 (1992), J. Biol. Chem., 267, 6807-6814.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene fabH, gene is located on the chromosome, DNA and amino acid sequence determination and analysis, functional complementation of enzyme-defective fabH1 mutant strain CDM5, expression in strain JT2038 alters the endogenous fatty acid composition | Escherichia coli K-12 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33517 | - |
x * 33517, DNA sequence calculation, x * 34800, recombinant enzyme, SDS-PAGE | Escherichia coli K-12 |
| 34800 | - |
x * 33517, DNA sequence calculation, x * 34800, recombinant enzyme, SDS-PAGE | Escherichia coli K-12 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | Escherichia coli K-12 | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli K-12 | P0A6R0 | gene fabH | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from strain JT2038 by ammonium sulfate fractionation, gel filtration, and affinity chromatography | Escherichia coli K-12 |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | the active site is formed by Ala-Cys-Ala | Escherichia coli K-12 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00013 | - |
acetyl transacetylase activity, recombinant strain JT2038 | Escherichia coli K-12 |
| 0.024 | - |
acetoacetyl-ACP synthase activity, recombinant strain JT2038 | Escherichia coli K-12 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Escherichia coli K-12 | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
r | |
| acetyl-CoA + [acyl-carrier protein] | the enzyme also shows acetyltransferase activity | Escherichia coli K-12 | acetyl-[acyl-carrier protein] + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33517, DNA sequence calculation, x * 34800, recombinant enzyme, SDS-PAGE | Escherichia coli K-12 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetoacetyl-ACP synthase | - |
Escherichia coli K-12 |
| acetyl-CoA:ACP transacylase | - |
Escherichia coli K-12 |
| ACP synthase III | - |
Escherichia coli K-12 |
| beta-ketoacyl-acyl carrier protein synthase III | - |
Escherichia coli K-12 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli K-12 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Escherichia coli K-12 |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli K-12 | DNA sequence calculation | - |
4.85 |
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