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Literature summary for 2.3.1.180 extracted from
- Crystal structure and substrate specificity of the beta-ketoacyl-acyl carrier protein synthase III (FabH) from Staphylococcus aureus (2005), Protein Sci., 14, 2087-2094.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of N-terminally His2-tagged enzyme in Escherichia coli | Staphylococcus aureus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| 15 mg/ml purified enzyme in 20 mM Tris, pH 8.0, 3 mM DTT, 100 mM NaCl, 10% glycerol, X-ray diffraction structure determination and analysis at 2.0 A resolution | Staphylococcus aureus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | 0.014 | isovaleryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus |  |
| 0.013 | 0.021 | hexanoyl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
| 0.019 | - |
acetyl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
| 0.02 | 0.022 | butyryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
| 0.024 | 0.037 | isobutyryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
2 * 36000, recombinant enzyme, SDS-PAGE and gel filtration | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | Staphylococcus aureus | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His2-tagged enzyme from Escherichia coli by nickel and Blue Sepharose affinity chromatography, and gel filtration | Staphylococcus aureus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | ping pong reaction mechanism, catalytically important residues are Phe298 and His238 | Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000001 | - |
purified recombinant enzyme, primer substrate acetyl-CoA | Staphylococcus aureus |
| 0.000003 | - |
purified recombinant enzyme, primer substrate lauroyl-CoA | Staphylococcus aureus |
| 0.000005 | - |
purified recombinant enzyme, primer substrate palmitoyl-CoA | Staphylococcus aureus |
| 0.000006 | - |
purified recombinant enzyme, primer substrate 2-methylcrotonyl-CoA | Staphylococcus aureus |
| 0.000013 | - |
purified recombinant enzyme, primer substrate propionyl-CoA | Staphylococcus aureus |
| 0.000015 | - |
purified recombinant enzyme, primer substrate isovaleryl-CoA | Staphylococcus aureus |
| 0.000022 | - |
purified recombinant enzyme, primer substrate butyryl-CoA | Staphylococcus aureus |
| 0.000024 | - |
purified recombinant enzyme, primer substrate hexanoyl-CoA | Staphylococcus aureus |
| 0.000031 | - |
purified recombinant enzyme, primer substrate isobutyryl-CoA | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylcrotonyl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | (4E)-4-methylhex-4-enoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| acetyl-CoA + malonyl-[acyl-carrier protein] | low activity | Staphylococcus aureus | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| butyryl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| hexanoyl-CoA + malonyl-[acyl-carrier protein] | high activity | Staphylococcus aureus | 3-oxo-octanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| isobutyryl-CoA + malonyl-[acyl-carrier protein] | isobutyryl-CoA is the preferred primer substrate | Staphylococcus aureus | 3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| isovaleryl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | 3-oxo-5-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| lauroyl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | 3-oxo-myristoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| additional information | the enzyme possesses a large primer binding pocket determining the substrate specificity | Staphylococcus aureus | ? | - |
? | |
| palmitoyl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | 3-oxo-stearoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| propionyl-CoA + malonyl-[acyl-carrier protein] | - |
Staphylococcus aureus | 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 36000, recombinant enzyme, SDS-PAGE and gel filtration | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-ketoacyl-acyl carrier protein synthase III | - |
Staphylococcus aureus |
| FabH | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Staphylococcus aureus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.13 | - |
acetyl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
| 3.8 | - |
butyryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
| 4.4 | 7.2 | acetyl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
| 8.1 | - |
hexanoyl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
| 15.5 | 30.5 | isovaleryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
| 20.9 | 41.6 | butyryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
| 25.5 | 41.7 | hexanoyl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
| 28 | 47.1 | isobutyryl-CoA | pH 7.0, 30°C, recombinant enzyme | Staphylococcus aureus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Staphylococcus aureus |
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