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Literature summary for 2.3.1.180 extracted from
- beta-ketoacyl-acyl carrier protein synthase III (FabH) is a determining factor in branched-chain fatty acid biosynthesis (2000), J. Bacteriol., 182, 365-370.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes fabH1 and fabH2, expression of His-tagged enzymes in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
genes fabH1 and fabH2 encoding isozymes FabH1 and FabH2 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificities of FabH1 and FabH2 | Bacillus subtilis |
| 0.024 | - |
purified recombinant His-tagged isoyzme FabH1, primer substrate acetyl-CoA | Bacillus subtilis |
| 0.105 | - |
purified recombinant His-tagged isoyzme FabH2, primer substrate acetyl-CoA | Bacillus subtilis |
| 0.155 | - |
purified recombinant His-tagged isoyzme FabH1, primer substrate 2-methylbutyryl-CoA | Bacillus subtilis |
| 0.162 | - |
purified recombinant His-tagged isoyzme FabH2, primer substrate isovaleryl-CoA | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylbutyryl-CoA + malonyl-[acyl-carrier protein] | 2-methylbutyryl-CoA is the preferred primer substrate of isozyme FabH1 | Bacillus subtilis | 3-oxo-4-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? |  |
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| butyryl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| heptanoyl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | 3-oxo-nonanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| hexanoyl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | 3-oxo-octanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| isobutyryl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | 3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| isovaleryl-CoA + malonyl-[acyl-carrier protein] | isovaleryl-CoA is the preferred primer substrate of isozyme FabH2 | Bacillus subtilis | 3-oxo-5-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| additional information | substrate specificities of FabH1 and FabH2 with different primer substrates, both isozymes prefer branched-chain fatty acids | Bacillus subtilis | ? | - |
? | |
| octanoyl-CoA + malonyl-[acyl-carrier protein] | low activity with octanoyl-CoA as primer substrate of isozyme FabH1, no activity with FabH2 | Bacillus subtilis | 3-oxo-decanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| propionyl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| valeryl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | 3-oxo-heptanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-ketoacyl-acyl carrier protein synthase III | - |
Bacillus subtilis |
| FabH | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Bacillus subtilis |
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