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Literature summary for 2.3.1.180 extracted from

  • Clough, R.; Matthis, A.L.; Barnum, S.R.; Jaworski, J.G.
    Purification and characterization of 3-ketoacyl-acyl carrier protein synthase III from spinach. A condensing enzyme utilizing acetyl-coenzyme A to initiate fatty acid synthesis (1992), J. Biol. Chem., 267, 20992-20998.
    View publication on PubMed

General Stability

General Stability Organism
0.01% Triton X-100 is essential for enzyme stability during purification Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
arsenite 45% inhibition at 5 mM, 98% inhibition at 20 mM Spinacia oleracea
N-ethylmaleimide complete inhibition at 5 mM Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40500
-
2 * 40500, SDS-PAGE Spinacia oleracea
63000
-
gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein] Spinacia oleracea
-
acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
r

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3500fold to homogeneity from leaves in an eight-step procedure including ACP affinity chromatography Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7
-
purified enzyme Spinacia oleracea

Storage Stability

Storage Stability Organism
-80°C, purified enzyme, 20 mM Tris buffer, 1 mM DTT, 20% glycerol, 0.01% Triton X-100, several weeks with slight loss of activity Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein]
-
Spinacia oleracea acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
r
additional information substrate specificity, the enzyme also shows acetyltransferase activity, which is lower than the 3-ketoacyl carrier protein synthase activity Spinacia oleracea ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40500, SDS-PAGE Spinacia oleracea

Synonyms

Synonyms Comment Organism
3-ketoacyl carrier protein synthase III
-
Spinacia oleracea
KAS-III
-
Spinacia oleracea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
assay at Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
CoA
-
Spinacia oleracea