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Literature summary for 2.3.1.179 extracted from

  • Zhu, L.; Cheng, J.; Luo, B.; Feng, S.; Lin, J.; Wang, S.; Cronan, J.E.; Wang, H.
    Functions of the Clostridium acetobutylicium FabF and FabZ proteins in unsaturated fatty acid biosynthesis (2009), BMC Microbiol., 9, 119.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene fabF1, expression in Escherichia coli strain BL21(DE3), and in strain K1060, a strain that carries an unconditional fabB allele, and in Escherichia coli strain CY242, which carries the same fabB(Ts) allele as strain CY244, but fabF1 fails to complement growth of the temperature sensitive fabB mutant strain CY242 at 42°C Clostridium acetobutylicum

Protein Variants

Protein Variants Comment Organism
additional information expression of Clostridium acetobutylicium FabF1 restores thermal control of fatty acid composition to an Escherichia coli FabF null mutant strain. FabF1 expression leads a modest conversion of cis-3-decenoyl-[acyl-carrier-protein] to trans-2-cis-5-dodecadienoyl-[acyl carrier-protein]. An Escherichia coli fabF- strain in which Clostridium acetobutylicium FabF1 is expressed from the lac promoter of a low copy number vector closely mimicks the changes in fatty acid composition seen in wild type Escherichia coli strains upon changes in growth temperature. Expression of Clostridium acetobutylicium FabF1 restores cis-vaccenate synthesis at all temperatures, but is much more effective at 30°C than at 37°C or 42°C Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium acetobutylicum FabF1 is able to catalyze all of the elongation reactions required in the synthesis of saturated fatty acids. The single 3-ketoacyl-[acyl-carrier-protein] synthase FabF of this bacterium performs the elongation functions required in both branches of the fatty acid synthetic pathway. The enzyme can both elongate palmitoleoyl-[acyl-carrier-protein] to cis-vaccenoyl-[acyl-carrier-protein] and elongate the cis double bond containing the product of FabA ?
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Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum
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gene fabF homologues designated as CAC3573, CAC2008 and CAA0093
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information FabF1 is able to catalyze all of the elongation reactions required in the synthesis of saturated fatty acids. The single 3-ketoacyl-[acyl-carrier-protein] synthase FabF of this bacterium performs the elongation functions required in both branches of the fatty acid synthetic pathway. The enzyme can both elongate palmitoleoyl-[acyl-carrier-protein] to cis-vaccenoyl-[acyl-carrier-protein] and elongate the cis double bond containing the product of FabA Clostridium acetobutylicum ?
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additional information of the enzymes encoded by fabF homologues designated as CAC3573, CAC2008 and CAA0093, only the first of these genes, fabF1, functions in fatty acid synthesis and can functionally replace Escherichia coli FabF in vivo, overview Clostridium acetobutylicum ?
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Synonyms

Synonyms Comment Organism
3-ketoacyl-ACP synthase
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Clostridium acetobutylicum
FabF1
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Clostridium acetobutylicum