BRENDA - Enzyme Database
show all sequences of 2.3.1.178

Genes and enzymes of ectoine biosynthesis in halotolerant methanotrophs

Reshetnikov, A.S.; Khmelenina, V.N.; Mustakhimov, I.I.; Trotsenko, Y.A.; Methods Enzymol. 495, 15-30 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)
Methylomicrobium alcaliphilum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cd2+
-
Methylomicrobium alcaliphilum
Cd2+
-
Methylophaga alcalica
Cd2+
-
Methylophaga thalassica
Cu2+
-
Methylophaga alcalica
Cu2+
-
Methylophaga thalassica
Zn2+
-
Methylomicrobium alcaliphilum
Zn2+
-
Methylophaga alcalica
Zn2+
-
Methylophaga thalassica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
acetyl-CoA
pH 9.5, 25°C
Methylophaga alcalica
0.037
-
acetyl-CoA
pH 9.5, 25°C
Methylomicrobium alcaliphilum
0.076
-
acetyl-CoA
pH 9.5, 25°C
Methylophaga thalassica
0.365
-
L-2,4-diaminobutanoate
pH 9.5, 25°C
Methylophaga thalassica
0.375
-
L-2,4-diaminobutanoate
pH 9.5, 25°C
Methylophaga alcalica
0.465
-
L-2,4-diaminobutanoate
pH 9.5, 25°C
Methylomicrobium alcaliphilum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
KCl
activates, best at 0.25 M
Methylomicrobium alcaliphilum
additional information
no activation by NaCl and KCl
Methylophaga alcalica
additional information
no activation by NaCl and KCl
Methylophaga thalassica
NaCl
activates, best at 0.1-0.2 M
Methylomicrobium alcaliphilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Methylomicrobium alcaliphilum
20000
-
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Methylophaga alcalica
20000
-
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Methylophaga thalassica
40000
-
recombinant His-tagged enzyme, gel filtration
Methylomicrobium alcaliphilum
40000
-
recombinant His-tagged enzyme, gel filtration
Methylophaga alcalica
40000
-
recombinant His-tagged enzyme, gel filtration
Methylophaga thalassica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + L-2,4-diaminobutanoate
Methylophaga thalassica
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
Halomonas elongata
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
Methylomicrobium alcaliphilum
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
Methylophaga alcalica
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
Methylomicrobium alcaliphilum 20Z
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Halomonas elongata
-
-
-
Methylomicrobium alcaliphilum
-
gene ectA
-
Methylomicrobium alcaliphilum 20Z
-
gene ectA
-
Methylophaga alcalica
-
gene ectA
-
Methylophaga thalassica
-
gene ectA
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
Methylomicrobium alcaliphilum
Storage Stability
Storage Stability
Organism
4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml
Methylophaga thalassica
4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml
Methylomicrobium alcaliphilum
4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml
Methylophaga alcalica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + L-2,4-diaminobutanoate
-
720373
Methylophaga thalassica
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
720373
Halomonas elongata
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
720373
Methylomicrobium alcaliphilum
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
720373
Methylophaga alcalica
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
720373
Methylomicrobium alcaliphilum 20Z
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
additional information
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
720373
Methylophaga thalassica
?
-
-
-
-
additional information
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
720373
Halomonas elongata
?
-
-
-
-
additional information
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
720373
Methylomicrobium alcaliphilum
?
-
-
-
-
additional information
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
720373
Methylophaga alcalica
?
-
-
-
-
additional information
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
720373
Methylomicrobium alcaliphilum 20Z
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Methylomicrobium alcaliphilum
homodimer
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Methylophaga alcalica
homodimer
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Methylophaga thalassica
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
-
Methylomicrobium alcaliphilum
25
-
assay at
Halomonas elongata
30
35
-
Methylophaga alcalica
30
35
-
Methylophaga thalassica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Halomonas elongata
8.5
-
-
Methylophaga thalassica
9.5
-
-
Methylomicrobium alcaliphilum
9.5
-
-
Methylophaga alcalica
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)
Methylomicrobium alcaliphilum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cd2+
-
Methylomicrobium alcaliphilum
Cd2+
-
Methylophaga alcalica
Cd2+
-
Methylophaga thalassica
Cu2+
-
Methylophaga alcalica
Cu2+
-
Methylophaga thalassica
Zn2+
-
Methylomicrobium alcaliphilum
Zn2+
-
Methylophaga alcalica
Zn2+
-
Methylophaga thalassica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
acetyl-CoA
pH 9.5, 25°C
Methylophaga alcalica
0.037
-
acetyl-CoA
pH 9.5, 25°C
Methylomicrobium alcaliphilum
0.076
-
acetyl-CoA
pH 9.5, 25°C
Methylophaga thalassica
0.365
-
L-2,4-diaminobutanoate
pH 9.5, 25°C
Methylophaga thalassica
0.375
-
L-2,4-diaminobutanoate
pH 9.5, 25°C
Methylophaga alcalica
0.465
-
L-2,4-diaminobutanoate
pH 9.5, 25°C
Methylomicrobium alcaliphilum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
KCl
activates, best at 0.25 M
Methylomicrobium alcaliphilum
additional information
no activation by NaCl and KCl
Methylophaga alcalica
additional information
no activation by NaCl and KCl
Methylophaga thalassica
NaCl
activates, best at 0.1-0.2 M
Methylomicrobium alcaliphilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Methylomicrobium alcaliphilum
20000
-
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Methylophaga alcalica
20000
-
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Methylophaga thalassica
40000
-
recombinant His-tagged enzyme, gel filtration
Methylomicrobium alcaliphilum
40000
-
recombinant His-tagged enzyme, gel filtration
Methylophaga alcalica
40000
-
recombinant His-tagged enzyme, gel filtration
Methylophaga thalassica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + L-2,4-diaminobutanoate
Methylophaga thalassica
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
Halomonas elongata
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
Methylomicrobium alcaliphilum
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
Methylophaga alcalica
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
Methylomicrobium alcaliphilum 20Z
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
Methylomicrobium alcaliphilum
Storage Stability (protein specific)
Storage Stability
Organism
4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml
Methylophaga thalassica
4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml
Methylomicrobium alcaliphilum
4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml
Methylophaga alcalica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + L-2,4-diaminobutanoate
-
720373
Methylophaga thalassica
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
720373
Halomonas elongata
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
720373
Methylomicrobium alcaliphilum
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
720373
Methylophaga alcalica
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
720373
Methylomicrobium alcaliphilum 20Z
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
additional information
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
720373
Methylophaga thalassica
?
-
-
-
-
additional information
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
720373
Halomonas elongata
?
-
-
-
-
additional information
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
720373
Methylomicrobium alcaliphilum
?
-
-
-
-
additional information
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
720373
Methylophaga alcalica
?
-
-
-
-
additional information
usage of 5,5-dithio-bis-(2-nitrobenzoic acid) for detection of activity
720373
Methylomicrobium alcaliphilum 20Z
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Methylomicrobium alcaliphilum
homodimer
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Methylophaga alcalica
homodimer
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE
Methylophaga thalassica
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
-
Methylomicrobium alcaliphilum
25
-
assay at
Halomonas elongata
30
35
-
Methylophaga alcalica
30
35
-
Methylophaga thalassica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Halomonas elongata
8.5
-
-
Methylophaga thalassica
9.5
-
-
Methylomicrobium alcaliphilum
9.5
-
-
Methylophaga alcalica
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the pathway of ectoine biosynthesis
Halomonas elongata
metabolism
the enzyme is involved in the pathway of ectoine biosynthesis
Methylomicrobium alcaliphilum
metabolism
the enzyme is involved in the pathway of ectoine biosynthesis
Methylophaga alcalica
metabolism
the enzyme is involved in the pathway of ectoine biosynthesis
Methylophaga thalassica
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the pathway of ectoine biosynthesis
Halomonas elongata
metabolism
the enzyme is involved in the pathway of ectoine biosynthesis
Methylomicrobium alcaliphilum
metabolism
the enzyme is involved in the pathway of ectoine biosynthesis
Methylophaga alcalica
metabolism
the enzyme is involved in the pathway of ectoine biosynthesis
Methylophaga thalassica
Other publictions for EC 2.3.1.178
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720373
Reshetnikov
Genes and enzymes of ectoine b ...
Halomonas elongata, Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum, Methylophaga alcalica, Methylophaga thalassica
Methods Enzymol.
495
15-30
2011
-
-
1
-
-
-
8
6
-
4
6
5
-
13
-
-
1
-
-
-
-
3
10
3
4
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
8
-
6
-
4
6
5
-
-
-
1
-
-
-
3
10
3
4
-
-
-
4
-
-
-
-
4
4
-
-
-
718536
Khmelenina
-
Genetic and biochemical aspect ...
Brevibacterium epidermidis, Chromohalobacter salexigens, Halobacillus halophilus, Halomonas elongata, Halomonas elongata KS3, Marinococcus halophilus, Methylomicrobium alcaliphilum, Methylophaga alcalica, Methylophaga thalassica, Sporosarcina pasteurii
Am. J. Agric. Biol. Sci.
5
446-458
2010
2
-
3
-
-
1
6
-
-
4
3
10
-
10
-
-
2
-
-
-
-
-
10
3
-
-
-
-
4
-
-
-
-
-
-
2
-
3
-
-
-
1
-
6
-
-
-
4
3
10
-
-
-
2
-
-
-
-
10
3
-
-
-
-
4
-
-
-
2
15
15
2
-
-
686806
Mustakhimov
Characterization of the recomb ...
Methylophaga alcalica, Methylophaga thalassica
FEMS Microbiol. Lett.
283
91-96
2008
-
-
2
-
-
-
12
2
-
-
3
2
-
5
-
-
2
-
-
-
-
2
2
2
2
-
-
-
2
2
-
-
-
-
-
-
-
2
-
-
-
-
-
12
-
2
-
-
3
2
-
-
-
2
-
-
-
2
2
2
2
-
-
-
2
2
-
-
-
-
-
-
-
-
660836
Reshetnikov
Characterization of the ectoin ...
Methylomicrobium alcaliphilum, Methylomicrobium alcaliphilum 20Z
Arch. Microbiol.
184
286-297
2006
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661224
Reshetnikov
Cloning, purification, and cha ...
Methylomicrobium alcaliphilum, Methylomicrobium alcaliphilum 20Z
Biochemistry (Moscow)
70
878-883
2005
-
-
1
-
-
-
3
2
-
2
2
-
-
9
-
-
1
-
-
-
-
1
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
2
-
2
2
-
-
-
-
1
-
-
-
1
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
660740
Kuhlmann
Osmotically regulated synthesi ...
Sporosarcina pasteurii
Appl. Environ. Microbiol.
68
772-783
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
11
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640091
Ono
Characterization of biosynthet ...
Halomonas elongata, Halomonas elongata OUT30018
J. Bacteriol.
181
91-99
1999
-
-
-
-
-
1
-
-
-
-
1
-
-
5
-
-
1
-
-
-
1
-
3
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
3
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
662773
Louis
Characterization of genes for ...
Marinococcus halophilus
Microbiology
143
1141-1149
1997
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640090
Peters
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The biosynthesis of ectoine ...
Halorhodospira halochloris
FEMS Microbiol. Lett.
71
157-162
1990
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