BRENDA - Enzyme Database
show all sequences of 2.3.1.178

Characterization of the recombinant diaminobutyric acid acetyltransferase from Methylophaga thalassica and Methylophaga alcalica

Mustakhimov, I.I.; Rozova, O.N.; Reshetnikov, A.S.; Khmelenina, V.N.; Murrell, J.C.; Trotsenko, Y.A.; FEMS Microbiol. Lett. 283, 91-96 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells
Methylophaga alcalica
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells
Methylophaga thalassica
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Methylophaga alcalica
Ca2+
-
Methylophaga thalassica
Cd2+
-
Methylophaga alcalica
Cd2+
-
Methylophaga thalassica
Cu2+
-
Methylophaga alcalica
Cu2+
-
Methylophaga thalassica
Mg2+
-
Methylophaga alcalica
Mg2+
-
Methylophaga thalassica
Ni2+
-
Methylophaga alcalica
Ni2+
-
Methylophaga thalassica
Zn2+
-
Methylophaga alcalica
Zn2+
-
Methylophaga thalassica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.37
-
2,4-diaminobutyrate
-
Methylophaga thalassica
0.375
-
2,4-diaminobutyrate
-
Methylophaga alcalica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19700
-
calculated molecular mass
Methylophaga thalassica
20000
-
calculated molecular mass; determined by gel filtration and SDS-PAGE
Methylophaga alcalica
20000
-
determined by gel filtration and SDS-PAGE
Methylophaga thalassica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + L-2,4-diaminobutanoate
Methylophaga thalassica
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
Methylophaga alcalica
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methylophaga alcalica
A9YZY0
-
-
Methylophaga thalassica
A9YZX5
-
-
Purification (Commentary)
Commentary
Organism
using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column
Methylophaga alcalica
using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column
Methylophaga thalassica
Storage Stability
Storage Stability
Organism
4C, 50 mM Tris-HCl, pH 8.5, 1 month
Methylophaga thalassica
4C, 50 mM Tris-HCl, pH 8.5, 1 month
Methylophaga alcalica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + L-2,4-diaminobutanoate
-
686806
Methylophaga thalassica
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
686806
Methylophaga alcalica
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
-
Methylophaga alcalica
homodimer
-
Methylophaga thalassica
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
activity assay
Methylophaga alcalica
30
-
activity assay
Methylophaga thalassica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Methylophaga thalassica
9.5
-
-
Methylophaga alcalica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10.5
-
Methylophaga alcalica
7
9.5
-
Methylophaga thalassica
Cloned(Commentary) (protein specific)
Commentary
Organism
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells
Methylophaga alcalica
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells
Methylophaga thalassica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
-
Methylophaga alcalica
Ca2+
-
Methylophaga thalassica
Cd2+
-
Methylophaga alcalica
Cd2+
-
Methylophaga thalassica
Cu2+
-
Methylophaga alcalica
Cu2+
-
Methylophaga thalassica
Mg2+
-
Methylophaga alcalica
Mg2+
-
Methylophaga thalassica
Ni2+
-
Methylophaga alcalica
Ni2+
-
Methylophaga thalassica
Zn2+
-
Methylophaga alcalica
Zn2+
-
Methylophaga thalassica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.37
-
2,4-diaminobutyrate
-
Methylophaga thalassica
0.375
-
2,4-diaminobutyrate
-
Methylophaga alcalica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19700
-
calculated molecular mass
Methylophaga thalassica
20000
-
calculated molecular mass; determined by gel filtration and SDS-PAGE
Methylophaga alcalica
20000
-
determined by gel filtration and SDS-PAGE
Methylophaga thalassica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + L-2,4-diaminobutanoate
Methylophaga thalassica
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
Methylophaga alcalica
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column
Methylophaga alcalica
using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column
Methylophaga thalassica
Storage Stability (protein specific)
Storage Stability
Organism
4C, 50 mM Tris-HCl, pH 8.5, 1 month
Methylophaga thalassica
4C, 50 mM Tris-HCl, pH 8.5, 1 month
Methylophaga alcalica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + L-2,4-diaminobutanoate
-
686806
Methylophaga thalassica
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
686806
Methylophaga alcalica
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
-
Methylophaga alcalica
homodimer
-
Methylophaga thalassica
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
activity assay
Methylophaga alcalica
30
-
activity assay
Methylophaga thalassica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Methylophaga thalassica
9.5
-
-
Methylophaga alcalica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10.5
-
Methylophaga alcalica
7
9.5
-
Methylophaga thalassica
Other publictions for EC 2.3.1.178
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720373
Reshetnikov
Genes and enzymes of ectoine b ...
Halomonas elongata, Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum, Methylophaga alcalica, Methylophaga thalassica
Methods Enzymol.
495
15-30
2011
-
-
1
-
-
-
8
6
-
4
6
5
-
13
-
-
1
-
-
-
-
3
10
3
4
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
8
-
6
-
4
6
5
-
-
-
1
-
-
-
3
10
3
4
-
-
-
4
-
-
-
-
4
4
-
-
-
718536
Khmelenina
-
Genetic and biochemical aspect ...
Brevibacterium epidermidis, Chromohalobacter salexigens, Halobacillus halophilus, Halomonas elongata, Halomonas elongata KS3, Marinococcus halophilus, Methylomicrobium alcaliphilum, Methylophaga alcalica, Methylophaga thalassica, Sporosarcina pasteurii
Am. J. Agric. Biol. Sci.
5
446-458
2010
2
-
3
-
-
1
6
-
-
4
3
10
-
10
-
-
2
-
-
-
-
-
10
3
-
-
-
-
4
-
-
-
-
-
-
2
-
3
-
-
-
1
-
6
-
-
-
4
3
10
-
-
-
2
-
-
-
-
10
3
-
-
-
-
4
-
-
-
2
15
15
2
-
-
686806
Mustakhimov
Characterization of the recomb ...
Methylophaga alcalica, Methylophaga thalassica
FEMS Microbiol. Lett.
283
91-96
2008
-
-
2
-
-
-
12
2
-
-
3
2
-
5
-
-
2
-
-
-
-
2
2
2
2
-
-
-
2
2
-
-
-
-
-
-
-
2
-
-
-
-
-
12
-
2
-
-
3
2
-
-
-
2
-
-
-
2
2
2
2
-
-
-
2
2
-
-
-
-
-
-
-
-
660836
Reshetnikov
Characterization of the ectoin ...
Methylomicrobium alcaliphilum, Methylomicrobium alcaliphilum 20Z
Arch. Microbiol.
184
286-297
2006
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661224
Reshetnikov
Cloning, purification, and cha ...
Methylomicrobium alcaliphilum, Methylomicrobium alcaliphilum 20Z
Biochemistry (Moscow)
70
878-883
2005
-
-
1
-
-
-
3
2
-
2
2
-
-
9
-
-
1
-
-
-
-
1
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
2
-
2
2
-
-
-
-
1
-
-
-
1
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
660740
Kuhlmann
Osmotically regulated synthesi ...
Sporosarcina pasteurii
Appl. Environ. Microbiol.
68
772-783
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
11
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640091
Ono
Characterization of biosynthet ...
Halomonas elongata, Halomonas elongata OUT30018
J. Bacteriol.
181
91-99
1999
-
-
-
-
-
1
-
-
-
-
1
-
-
5
-
-
1
-
-
-
1
-
3
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
3
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
662773
Louis
Characterization of genes for ...
Marinococcus halophilus
Microbiology
143
1141-1149
1997
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640090
Peters
-
The biosynthesis of ectoine ...
Halorhodospira halochloris
FEMS Microbiol. Lett.
71
157-162
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-