Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli with an additional six His residues at the C-terminus | Methylotuvimicrobium alcaliphilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 10 mM, 26% inhibition, 1 mM, 18% inhibition | Methylotuvimicrobium alcaliphilum | |
Cd2+ | 1 mM, complete inhibition | Methylotuvimicrobium alcaliphilum | |
Zn2+ | 1 mM, complete inhibition | Methylotuvimicrobium alcaliphilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0366 | - |
acetyl-CoA | - |
Methylotuvimicrobium alcaliphilum | |
0.46 | - |
L-2,4-diaminobutanoate | - |
Methylotuvimicrobium alcaliphilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | 0.25 M, activity increases threefold | Methylotuvimicrobium alcaliphilum | |
NaCl | 0.1-0.15 M, activity increases threefold | Methylotuvimicrobium alcaliphilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
2 * 20000, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
40000 | - |
gel filtration | Methylotuvimicrobium alcaliphilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylotuvimicrobium alcaliphilum | - |
20Z | - |
Methylotuvimicrobium alcaliphilum 20Z | - |
20Z | - |
Purification (Comment) | Organism |
---|---|
- |
Methylotuvimicrobium alcaliphilum |
Storage Stability | Organism |
---|---|
4°C or -70°C, protein concentration 0.5 mg/ml, stable for at least 1 month in 50 mM Tris-HCl, pH 8.5, containing 0.2 M KCl | Methylotuvimicrobium alcaliphilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylotuvimicrobium alcaliphilum | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylotuvimicrobium alcaliphilum 20Z | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
propionyl-CoA + L-2,4-diaminobutanoate | 20% of the activity with acetyl-CoA | Methylotuvimicrobium alcaliphilum | CoA + N4-propionyl-L-2,4-diaminobutanoate | - |
? | |
propionyl-CoA + L-2,4-diaminobutanoate | 20% of the activity with acetyl-CoA | Methylotuvimicrobium alcaliphilum 20Z | CoA + N4-propionyl-L-2,4-diaminobutanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 20000, SDS-PAGE | Methylotuvimicrobium alcaliphilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Methylotuvimicrobium alcaliphilum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 25 | 15°C: about 35% of maximal activity, 25°C: about 65% of maximal activity | Methylotuvimicrobium alcaliphilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Methylotuvimicrobium alcaliphilum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10.5 | pH 8: about 50% of maximal activity, pH 10.5: about 70% of maximal activity | Methylotuvimicrobium alcaliphilum |