BRENDA - Enzyme Database
show all sequences of 2.3.1.178

Cloning, purification, and characterization of diaminobutyrate acetyltransferase from the halotolerant methanotroph Methylomicrobium alcaliphilum 20Z

Reshetnikov, A.S.; Mustakhimov, II; Khmelenina, V.N.; Trotsenko, Y.A.; Biochemistry (Moscow) 70, 878-883 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli with an additional six His residues at the C-terminus
Methylomicrobium alcaliphilum
Inhibitors
Inhibitors
Commentary
Organism
Structure
ATP
10 mM, 26% inhibition, 1 mM, 18% inhibition
Methylomicrobium alcaliphilum
Cd2+
1 mM, complete inhibition
Methylomicrobium alcaliphilum
Zn2+
1 mM, complete inhibition
Methylomicrobium alcaliphilum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0366
-
acetyl-CoA
-
Methylomicrobium alcaliphilum
0.46
-
L-2,4-diaminobutanoate
-
Methylomicrobium alcaliphilum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
KCl
0.25 M, activity increases threefold
Methylomicrobium alcaliphilum
NaCl
0.1-0.15 M, activity increases threefold
Methylomicrobium alcaliphilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
2 * 20000, SDS-PAGE
Methylomicrobium alcaliphilum
40000
-
gel filtration
Methylomicrobium alcaliphilum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methylomicrobium alcaliphilum
-
20Z
-
Methylomicrobium alcaliphilum 20Z
-
20Z
-
Purification (Commentary)
Commentary
Organism
-
Methylomicrobium alcaliphilum
Storage Stability
Storage Stability
Organism
4C or -70C, protein concentration 0.5 mg/ml, stable for at least 1 month in 50 mM Tris-HCl, pH 8.5, containing 0.2 M KCl
Methylomicrobium alcaliphilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + L-2,4-diaminobutanoate
-
661224
Methylomicrobium alcaliphilum
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
661224
Methylomicrobium alcaliphilum 20Z
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
propionyl-CoA + L-2,4-diaminobutanoate
20% of the activity with acetyl-CoA
661224
Methylomicrobium alcaliphilum
CoA + N4-propionyl-L-2,4-diaminobutanoate
-
-
-
?
propionyl-CoA + L-2,4-diaminobutanoate
20% of the activity with acetyl-CoA
661224
Methylomicrobium alcaliphilum 20Z
CoA + N4-propionyl-L-2,4-diaminobutanoate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 20000, SDS-PAGE
Methylomicrobium alcaliphilum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
20
-
-
Methylomicrobium alcaliphilum
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
15
25
15C: about 35% of maximal activity, 25C: about 65% of maximal activity
Methylomicrobium alcaliphilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Methylomicrobium alcaliphilum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
10.5
pH 8: about 50% of maximal activity, pH 10.5: about 70% of maximal activity
Methylomicrobium alcaliphilum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli with an additional six His residues at the C-terminus
Methylomicrobium alcaliphilum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ATP
10 mM, 26% inhibition, 1 mM, 18% inhibition
Methylomicrobium alcaliphilum
Cd2+
1 mM, complete inhibition
Methylomicrobium alcaliphilum
Zn2+
1 mM, complete inhibition
Methylomicrobium alcaliphilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0366
-
acetyl-CoA
-
Methylomicrobium alcaliphilum
0.46
-
L-2,4-diaminobutanoate
-
Methylomicrobium alcaliphilum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
KCl
0.25 M, activity increases threefold
Methylomicrobium alcaliphilum
NaCl
0.1-0.15 M, activity increases threefold
Methylomicrobium alcaliphilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
2 * 20000, SDS-PAGE
Methylomicrobium alcaliphilum
40000
-
gel filtration
Methylomicrobium alcaliphilum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methylomicrobium alcaliphilum
Storage Stability (protein specific)
Storage Stability
Organism
4C or -70C, protein concentration 0.5 mg/ml, stable for at least 1 month in 50 mM Tris-HCl, pH 8.5, containing 0.2 M KCl
Methylomicrobium alcaliphilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + L-2,4-diaminobutanoate
-
661224
Methylomicrobium alcaliphilum
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
661224
Methylomicrobium alcaliphilum 20Z
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
-
-
?
propionyl-CoA + L-2,4-diaminobutanoate
20% of the activity with acetyl-CoA
661224
Methylomicrobium alcaliphilum
CoA + N4-propionyl-L-2,4-diaminobutanoate
-
-
-
?
propionyl-CoA + L-2,4-diaminobutanoate
20% of the activity with acetyl-CoA
661224
Methylomicrobium alcaliphilum 20Z
CoA + N4-propionyl-L-2,4-diaminobutanoate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 20000, SDS-PAGE
Methylomicrobium alcaliphilum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
20
-
-
Methylomicrobium alcaliphilum
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
15
25
15C: about 35% of maximal activity, 25C: about 65% of maximal activity
Methylomicrobium alcaliphilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Methylomicrobium alcaliphilum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
10.5
pH 8: about 50% of maximal activity, pH 10.5: about 70% of maximal activity
Methylomicrobium alcaliphilum
Other publictions for EC 2.3.1.178
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720373
Reshetnikov
Genes and enzymes of ectoine b ...
Halomonas elongata, Methylomicrobium alcaliphilum 20Z, Methylomicrobium alcaliphilum, Methylophaga alcalica, Methylophaga thalassica
Methods Enzymol.
495
15-30
2011
-
-
1
-
-
-
8
6
-
4
6
5
-
13
-
-
1
-
-
-
-
3
10
3
4
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
8
-
6
-
4
6
5
-
-
-
1
-
-
-
3
10
3
4
-
-
-
4
-
-
-
-
4
4
-
-
-
718536
Khmelenina
-
Genetic and biochemical aspect ...
Brevibacterium epidermidis, Chromohalobacter salexigens, Halobacillus halophilus, Halomonas elongata, Halomonas elongata KS3, Marinococcus halophilus, Methylomicrobium alcaliphilum, Methylophaga alcalica, Methylophaga thalassica, Sporosarcina pasteurii
Am. J. Agric. Biol. Sci.
5
446-458
2010
2
-
3
-
-
1
6
-
-
4
3
10
-
10
-
-
2
-
-
-
-
-
10
3
-
-
-
-
4
-
-
-
-
-
-
2
-
3
-
-
-
1
-
6
-
-
-
4
3
10
-
-
-
2
-
-
-
-
10
3
-
-
-
-
4
-
-
-
2
15
15
2
-
-
686806
Mustakhimov
Characterization of the recomb ...
Methylophaga alcalica, Methylophaga thalassica
FEMS Microbiol. Lett.
283
91-96
2008
-
-
2
-
-
-
12
2
-
-
3
2
-
5
-
-
2
-
-
-
-
2
2
2
2
-
-
-
2
2
-
-
-
-
-
-
-
2
-
-
-
-
-
12
-
2
-
-
3
2
-
-
-
2
-
-
-
2
2
2
2
-
-
-
2
2
-
-
-
-
-
-
-
-
660836
Reshetnikov
Characterization of the ectoin ...
Methylomicrobium alcaliphilum, Methylomicrobium alcaliphilum 20Z
Arch. Microbiol.
184
286-297
2006
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661224
Reshetnikov
Cloning, purification, and cha ...
Methylomicrobium alcaliphilum, Methylomicrobium alcaliphilum 20Z
Biochemistry (Moscow)
70
878-883
2005
-
-
1
-
-
-
3
2
-
2
2
-
-
9
-
-
1
-
-
-
-
1
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
2
-
2
2
-
-
-
-
1
-
-
-
1
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
660740
Kuhlmann
Osmotically regulated synthesi ...
Sporosarcina pasteurii
Appl. Environ. Microbiol.
68
772-783
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
11
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640091
Ono
Characterization of biosynthet ...
Halomonas elongata, Halomonas elongata OUT30018
J. Bacteriol.
181
91-99
1999
-
-
-
-
-
1
-
-
-
-
1
-
-
5
-
-
1
-
-
-
1
-
3
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
3
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
662773
Louis
Characterization of genes for ...
Marinococcus halophilus
Microbiology
143
1141-1149
1997
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640090
Peters
-
The biosynthesis of ectoine ...
Halorhodospira halochloris
FEMS Microbiol. Lett.
71
157-162
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-