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Literature summary for 2.3.1.177 extracted from

  • Stewart, C.; Woods, K.; Macias, G.; Allan, A.C.; Hellens, R.P.; Noel, J.P.
    Molecular architectures of benzoic acid-specific type III polyketide synthases (2017), Acta Crystallogr. Sect. D, 73, 1007-1019 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method using buffered protein solutions (10-15 mg/ml) mixed with equal volumes of the reservoir solution and incubated at 4°C. Crystal structures of biphenyl synthase from Malus domestica and benzophenone synthase from Hypericum androsaemum are compared with the structure of an archetypal type III polyketide synthase - chalcone synthase from Malus domestica Malus domestica
hanging-drop vapor-diffusion method, incubation at 4°C. Crystal structures of biphenyl synthase from Malusx02domestica and benzophenone synthase from Hypericum androsaemum are compared with the structure of an archetypal type III polyketide synthase: chalcone synthase from Malus domestica. Both biphenyl synthase and benzophenone synthase contain mutations that reshape their active-site cavities to prevent the binding of 4-coumaroyl-CoA and to favor the binding of small hydrophobic substrates. The active-site cavities of biphenyl synthase and benzophenone synthase also contain a novel pocket associated with their chain-elongation and cyclization reactions Malus domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
benzoyl-CoA pH 7.0, 37°C Malus domestica
0.00068
-
malonyl-CoA pH 7.0, 37°C Malus domestica
0.0008
-
benzoyl-CoA pH 7.0, 37°C Malus domestica
0.0041
-
malonyl-CoA pH 7.0, 37°C Malus domestica
0.0068
-
malonyl-CoA pH 7.0, 37°C Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 malonyl-CoA + benzoyl-CoA Malus domestica
-
4 CoA + 3,5-dihydroxybiphenyl + 4 CO2
-
?

Organism

Organism UniProt Comment Textmining
Malus domestica K9MS05
-
-
Malus domestica K9MST3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 malonyl-CoA + benzoyl-CoA
-
Malus domestica 4 CoA + 3,5-dihydroxybiphenyl + 4 CO2
-
?

Synonyms

Synonyms Comment Organism
BIS1 biphenyl synthase
-
Malus domestica
BIS3 biphenyl synthase
-
Malus domestica
MdBIS1
-
Malus domestica
MdBIS3
-
Malus domestica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.135
-
benzoyl-CoA pH 7.0, 37°C Malus domestica
0.283
-
benzoyl-CoA pH 7.0, 37°C Malus domestica

General Information

General Information Comment Organism
evolution crystal structures of biphenyl synthase from Malus domestica and benzophenone synthase from Hypericum androsaemum are compared with the structure of an archetypal type III polyketide synthase - chalcone synthase from Malus domestica. The results illuminate structural determinants of benzoic acid-specific type III PKSs and expand the understanding of the evolution of specialized metabolic pathways in plants Malus domestica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
270
-
benzoyl-CoA pH 7.0, 37°C Malus domestica
350
-
benzoyl-CoA pH 7.0, 37°C Malus domestica
353.75
-
benzoyl-CoA pH 7.0, 37°C Malus domestica