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Literature summary for 2.3.1.175 extracted from

  • Lejon, S.; Ellis, J.; Valegard, K.
    The last step in cephalosporin C formation revealed: crystal structures of deacetylcephalosporin C acetyltransferase from Acremonium chrysogenum in complexes with reaction intermediates (2008), J. Mol. Biol., 377, 935-944.
    View publication on PubMed
Search for more literature for 2.3.1.175

Application

Application Comment Organism
biotechnology studies of the enzyme will be useful in gaining a full view of the possibilities for overcoming the acetyl transfer bottleneck and for the future development of cephalosporin antibiotics Acremonium chrysogenum

Cloned(Commentary)

Cloned (Comment) Organism
into the pTWIN vector for expression in Escherichia coli BL21DE3 cells Acremonium chrysogenum

Crystallization (Commentary)

Crystallization (Comment) Organism
different structures are solved, SeMet-DAC-AT with acetyl-CoA to a resolution of 2.4 A, DAC-AT with acetyl-CoA to 2.2 A, DAC-AT with DAC/cephalosporin C to 2.5 A, and DAC-AT with DAC to 2.6 A resolution Acremonium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + deacetylcephalosporin C Acremonium chrysogenum
-
 CoA + cephalosporin C
-
 ?

Organism

Organism UniProt Comment Textmining
Acremonium chrysogenum P39058
-
-

Purification (Commentary)

Purification (Comment) Organism
using a gravity-flow column containing chitin beads, cleavage of the chitin-binding domain and intein fusion partner is performed Acremonium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + deacetylcephalosporin C
-
 Acremonium chrysogenum CoA + cephalosporin C
-
 ?

Synonyms

Synonyms Comment Organism
DAC-AT
-
 Acremonium chrysogenum
deacetylcephalosporin-C acetyltransferase
-
 Acremonium chrysogenum