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Literature summary for 2.3.1.175 extracted from

  • Ullan, R.V.; Campoy, S.; Casqueiro, J.; Fernandez, F.J.; Martin, J.F.
    Deacetylcephalosporin C production in Penicillium chrysogenum by expression of the isopenicillin N epimerization, ring expansion, and acetylation genes (2007), Chem. Biol., 14, 329-339.
    View publication on PubMed

Application

Application Comment Organism
biotechnology Penicillium chrysogenum npe6 lacking isopenicillin N acyltransferase activity is an excellent host for production of different beta-lactam antibiotics Acremonium chrysogenum
synthesis production of acetylcephalosporin C by gene expression in Penicillium chrysogenum. Recombinant strains secrete significant amounts of deacetylcephalosporin C, but acetylcephalosporin C is not detected in culture broth. Even when accumulating intracellularly, acetylcephalosporin C is not found extracellularly Acremonium chrysogenum

Cloned(Commentary)

Cloned (Comment) Organism
Penicillium chrysogenum strains expressing cefD1, cefD2, cefEF, and cefG genes cloned form Acremonium chrysogenum are constructed, deacetylcephalosporin-C acetyltransferase is coded by the cefG gene Acremonium chrysogenum

Localization

Localization Comment Organism GeneOntology No. Textmining
protoplast
-
Acremonium chrysogenum
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + deacetylcephalosporin C Acremonium chrysogenum
-
CoA + cephalosporin C
-
?

Organism

Organism UniProt Comment Textmining
Acremonium chrysogenum
-
-
-
Acremonium chrysogenum
-
expression in Penicillium chrysogenum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.7
-
deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA64 Acremonium chrysogenum
7.2
-
deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA71 Acremonium chrysogenum
25.8
-
deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA2 Acremonium chrysogenum
30
-
deacetylcephalosporin-C acetyltransferase activity in the host strain Penicillium chrysogenum TA98 Acremonium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + deacetylcephalosporin C
-
Acremonium chrysogenum CoA + cephalosporin C
-
?

Synonyms

Synonyms Comment Organism
acetyl-CoA:DAC-acetyltransferase
-
Acremonium chrysogenum
deacetylcephalosporin-C acetyltransferase
-
Acremonium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
activity assay Acremonium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
activity assay Acremonium chrysogenum