Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.174 extracted from

  • Kaschabek, S.R.; Kuhn, B.; Muller, D.; Schmidt, E.; Reineke, W.
    Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase (2002), J. Bacteriol., 184, 207-215.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.174

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 1 mM, 90% inhibition Pseudomonas sp.
additional information 1 mM EDTA or 1,10-phenanthroline have no effect Pseudomonas sp.
NaCN 1 mM, 12% inhibition Pseudomonas sp.
NADH 10% inhibition with 0.4 mM, 42% inhibition by 0.8 mM Pseudomonas sp.
ZnCl2 1 mM, 46% inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 CoA
-
 Pseudomonas sp.
0.15
-
 3-oxoadipyl-CoA
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 4 * 42000, SDS-PAGE Pseudomonas sp.
162000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. B13
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.1
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + 3-oxoadipyl-CoA
-
 Pseudomonas sp. succinyl-CoA + acetyl-CoA
-
 ?
CoA + 3-oxoadipyl-CoA
-
 Pseudomonas sp. B13 succinyl-CoA + acetyl-CoA
-
 ?
CoA + oxoadipyl-CoA
-
 Pseudomonas sp. succinyl-CoA + acetyl-CoA
-
 ?
CoA + oxoadipyl-CoA
-
 Pseudomonas sp. B13 succinyl-CoA + acetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 42000, SDS-PAGE Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.8
-
 CoA
-
 Pseudomonas sp.
7.8
-
 3-oxoadipyl-CoA
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Pseudomonas sp.