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Literature summary for 2.3.1.172 extracted from

  • DAuria, J.C.; Reichelt, M.; Luck, K.; Svatos, A.; Gershenzon, J.
    Identification and characterization of the BAHD acyltransferase malonyl CoA: anthocyanidin 5-O-glucoside-6-O-malonyltransferase (At5MAT) in Arabidopsis thaliana (2007), FEBS Lett., 581, 872-878.
    View publication on PubMed
Search for more literature for 2.3.1.172

Cloned(Commentary)

Cloned (Comment) Organism
into the entry vector pDONR207, the entry clone is used to transfer the open reading frame into the expression plasmid pH9GW for expression of the protein in Escherichia coli Bl21DE3 cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 malonyl-CoA cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside as co-substrate, pH 7.1, 25°C Arabidopsis thaliana
0.0076
-
 cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside pH 7.1, 25°C Arabidopsis thaliana
0.0153
-
 malonyl-CoA cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside as co-substrate, pH 7.1, 25°C Arabidopsis thaliana
0.07
-
 cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside pH 7.1, 25°C Arabidopsis thaliana
0.167
-
 cyanin pH 7.1, 25°C Arabidopsis thaliana
2.26
-
 malvin pH 7.1, 25°C Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49550
-
 predicted molecular mass Arabidopsis thaliana
55350
-
 predicted molecular mass of the fusion protein Arabidopsis thaliana
57000
-
 determined by SDS-PAGE, fusion protein, At5MAT His-tagged Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside Arabidopsis thaliana
-
 CoA + cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
malonyl-CoA + cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside Arabidopsis thaliana
-
 CoA + cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
malonyl-CoA + cyanin Arabidopsis thaliana
-
 CoA + cyanidin 3-O-beta-D-glucoside 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
malonyl-CoA + malvin Arabidopsis thaliana
-
 CoA + malvidin 3-O-beta-D-glucoside 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LJB4
-
-

Purification (Commentary)

Purification (Comment) Organism
using His-tag affinity chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside
-
 Arabidopsis thaliana CoA + cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
malonyl-CoA + cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside
-
 Arabidopsis thaliana CoA + cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
malonyl-CoA + cyanin
-
 Arabidopsis thaliana CoA + cyanidin 3-O-beta-D-glucoside 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
malonyl-CoA + malvin
-
 Arabidopsis thaliana CoA + malvidin 3-O-beta-D-glucoside 5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
At5MAT
-
 Arabidopsis thaliana
malonyl CoA:anthocyanidin 5-O-glucoside-6"-O-malonyltransferase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 enzyme assay Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00012
-
 cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside pH 7.1, 25°C Arabidopsis thaliana
0.0013
-
 malonyl-CoA cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside as co-substrate, pH 7.1, 25°C Arabidopsis thaliana
0.02
-
 cyanin pH 7.1, 25°C Arabidopsis thaliana
0.12
-
 cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside pH 7.1, 25°C Arabidopsis thaliana
0.2
-
 malonyl-CoA cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside as co-substrate, pH 7.1, 25°C Arabidopsis thaliana
1.79
-
 malvin pH 7.1, 25°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
 enzyme assay Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5
-
 Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.016
-
 cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside pH 7.1, 25°C Arabidopsis thaliana
0.12
-
 cyanin pH 7.1, 25°C Arabidopsis thaliana
0.62
-
 malonyl-CoA cyanidin 3-O-[[2-O-(2-O-sinapoyl)-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside as co-substrate, pH 7.1, 25°C Arabidopsis thaliana
0.79
-
 malvin pH 7.1, 25°C Arabidopsis thaliana
1.71
-
 cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside pH 7.1, 25°C Arabidopsis thaliana
13.1
-
 malonyl-CoA cyanidin 3-O-[[2-O-beta-D-xylosyl]-6-O-(6-O-(4-coumaroyl)-beta-D-glucoside)] 5-O-beta-D-glucoside as co-substrate, pH 7.1, 25°C Arabidopsis thaliana