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Literature summary for 2.3.1.17 extracted from

  • Tahay, G.; Wiame, E.; Tyteca, D.; Courtoy, P.J.; Van Schaftingen, E.
    Determinants of the enzymatic activity and the subcellular localization of aspartate N-acetyltransferase (2012), Biochem. J., 441, 105-112.
    View publication on PubMed
Search for more literature for 2.3.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK-293T cell Homo sapiens

Protein Variants

Protein Variants Comment Organism
C128A mutation in region 4, 94% of wild-type expression, 88% of wild-type activity Homo sapiens
C139A mutation in region 4, 70% of wild-type expression, 126% of wild-type activity Homo sapiens
D168A mutation in region 5, 46% of wild-type expression, no residual activity Homo sapiens
D168E mutation in region 5, 63% of wild-type expression, 7% of wild-type activity Homo sapiens
E101A mutation in region 3, 97% of wild-type expression, 42% of wild-type activity Homo sapiens
E101D mutation in region 3, 61% of wild-type expression, 99% of wild-type activity Homo sapiens
additional information transfection of truncated forms of isoform NAT8L into HEK-293T cells indicates that the 68 N-terminal residues, i.e. regions 1 and 2, have no importance for the catalytic activity and the subcellular localization of the enzyme. The membrane region, i.e. region 4, is necessary and sufficient to target isoform NAT8L to the endoplasmic reticulum Homo sapiens
P142A mutation in region 4, 113% of wild-type expression, 116% of wild-type activity Homo sapiens
R133A mutation in region 4, 78% of wild-type expression, 64% of wild-type activity Homo sapiens
R133K mutation in region 4, 69% of wild-type expression, 89% of wild-type activity Homo sapiens
R220A mutation in region 5, 101% of wild-type expression, 14% of wild-type activity Homo sapiens
R220K mutation in region 5, 104% of wild-type expression, 25% of wild-type activity Homo sapiens
R81A mutation in region 3, 57% of wild-type expression, 37% of wild-type activity Homo sapiens
R81K mutation in region 3, 90% of wild-type expression, 82% of wild-type activity Homo sapiens
S132F/R133F mutation in region 4, 92% of wild-type expression, 41% of wild-type activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 acetyl-CoA wild-type, pH 7.1, 30°C Homo sapiens
0.012
-
 acetyl-CoA mutant P142A, pH 7.1, 30°C Homo sapiens
0.017
-
 acetyl-CoA mutant S132F/R133F, pH 7.1, 30°C Homo sapiens
0.019
-
 acetyl-CoA mutant R133A, pH 7.1, 30°C Homo sapiens
0.082
-
 acetyl-CoA mutant R81A, pH 7.1, 30°C Homo sapiens
0.09
-
 L-aspartate wild-type, pH 7.1, 30°C Homo sapiens
0.095
-
 L-aspartate mutant P142A, pH 7.1, 30°C Homo sapiens
0.127
-
 acetyl-CoA mutant E101A, pH 7.1, 30°C Homo sapiens
0.128
-
 L-aspartate mutant S132F/R133F, pH 7.1, 30°C Homo sapiens
0.14
-
 acetyl-CoA mutant R220K, pH 7.1, 30°C Homo sapiens
0.2
-
 L-aspartate mutant R81A, pH 7.1, 30°C Homo sapiens
0.4
-
 acetyl-CoA mutant R220A, pH 7.1, 30°C Homo sapiens
0.434
-
 L-aspartate mutant E101A, pH 7.1, 30°C Homo sapiens
1.61
-
 L-aspartate mutant R220K, pH 7.1, 30°C Homo sapiens
3.37
-
 L-aspartate mutant R220A, pH 7.1, 30°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum exclusively associated with the endoplasmic reticulum. the membrane region comprises alpha-helices and the catalytic site is in the cytosol. The membrane region, i.e. region 4, is necessary and sufficient to target isoform NAT8L to the endoplasmic reticulum Homo sapiens 5783
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8N9F0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-aspartate
-
 Homo sapiens CoA + N-acetyl-L-aspartic acid
-
 ?

Synonyms

Synonyms Comment Organism
NAT8L
-
 Homo sapiens