Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CHAPS | 40% of activity at 12 mM, 10% of activity at 20 mM | Rattus norvegicus | |
CoA | IC50: 0.42 mM | Rattus norvegicus | |
N-acetyl-L-aspartic acid | IC50: 0.85 mM | Rattus norvegicus | |
NaCl | above 150 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
acetyl-CoA | pH 7.1, 37°C | Rattus norvegicus | |
0.58 | - |
L-aspartate | pH 7.1, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
670000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-aspartate | Rattus norvegicus | decreases in enzyme activity occur in a number of neurological disorders, possible role in neuronal mitochondrial energy metabolism | CoA + N-acetyl-L-aspartic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
neuron | - |
Rattus norvegicus | - |
oligodendrocyte | cultured | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-aspartate | - |
Rattus norvegicus | CoA + N-acetyl-L-aspartic acid | - |
? | |
acetyl-CoA + L-aspartate | decreases in enzyme activity occur in a number of neurological disorders, possible role in neuronal mitochondrial energy metabolism | Rattus norvegicus | CoA + N-acetyl-L-aspartic acid | - |
? | |
additional information | highly specific for L-aspartate with 3% or less active for L-glutamate, L-asparagine, L-glutamine, or aspartate-glutamate dipeptide | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | at least 10 bands on SDS-PAGE indicating a large enzyme complex | Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.42 | - |
IC50: 0.42 mM | Rattus norvegicus | CoA | |
0.85 | - |
IC50: 0.85 mM | Rattus norvegicus | N-acetyl-L-aspartic acid |