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Literature summary for 2.3.1.168 extracted from
- Invitro reconstitution of the 24-meric E2 inner core of bovine mitochondrial branched-chain alpha-keto acid dehydrogenase complex: requirements for chaperonins GroEL and GroES (1994), Biochemistry, 33, 8962-8968.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of E2c, residues 161-421, fused to maltose-binding protein, which enhances the yield of the recombinant enzyme, in Escherichia coli | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
E2-trimer, sucrose density gradient centrifugation in presence of the chaotropic reagent guanidinium-HCl | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant E2c, residues 161-421, fused to maltose-binding protein from Escherichia coli | Bos taurus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| E2c, completely unfolded in 4.5 M guanidinium chloride, is diluted 100fold at 25°C and refolded in 5 mM MgATP2- and a 4fold molar excess of chaperonins GroEL and GroES at pH 7.5, full activity is recovered after 45 min, an active GroEL-E2 24-mer is formed | Bos taurus |
| in vitro reconstitution of the 24-meric E2 inner core requires the chaperonins GroEL and GroES | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine | - |
Bos taurus | CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | in vitro reconstitution of the 24-meric E2 inner core requires the chaperonins GroEL and GroES | Bos taurus |
| More | the 24-mer can be separated into active trimers of MW 84 kDa by incubation in 1.5 M guanidinium-HCl at 25°C, process is reversible, and removal of guanidinium-HCl leads to spontenaous reassembly to an active 24-mer | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme is the E2 component of the multienzyme complex branched-chain alpha-keto acid dehydrogenase, i.e. branched-chain 2-oxo acid dehydrogenase | Bos taurus |
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