Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.168 extracted from

  • Hakozaki, M.; Ono, K.; Suzuki, T.; Hata, H.; Mori, T.; Kochi, H.
    Characterization of rainbow trout branched-chain alpha-keto acid dehydrogenase complex: inter-domain segments of the E2 component affect the overall activity (2002), Comp. Biochem. Physiol. B Biochem. Mol. Biol., 132, 433-442.
    View publication on PubMed
Search for more literature for 2.3.1.168

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination anad analysis Oncorhynchus mykiss

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Oncorhynchus mykiss

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Oncorhynchus mykiss 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, SDS-PAGE Oncorhynchus mykiss

Organism

Organism UniProt Comment Textmining
Gallus gallus Q98UJ6
-
-
Oncorhynchus mykiss Q8QHL7 rainbow trout
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oncorhynchus mykiss

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of the multienzyme complex branched-chain alpha-keto dehydrogenase Oncorhynchus mykiss

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1
-
 purified enzyme, as part of the multienzyme complex, substrate isovaleryl-CoA Oncorhynchus mykiss

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine
-
 Oncorhynchus mykiss CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine
-
 ?
2-oxoglutaryl-CoA + dihydrolipoamide low activity Oncorhynchus mykiss CoA + S-(2-oxoglutaryl)dihydrolipoamide
-
 ?
3-methylisovaleryl-CoA + dihydrolipoamide
-
 Oncorhynchus mykiss CoA + S-(3-methylisovaleryl)dihydrolipoamide
-
 ?
4-methylthiobutyryl-CoA + dihydrolipoamide low activity Oncorhynchus mykiss CoA + S-(4-methylthiobutyryl)dihydrolipoamide
-
 ?
butyryl-CoA + dihydrolipoamide
-
 Oncorhynchus mykiss CoA + S-butyryldihydrolipoamide
-
 ?
isocaproyl-CoA + dihydrolipoamide
-
 Oncorhynchus mykiss CoA + S-isohexanoyldihydrolipoamide
-
 ?
isovaleryl-CoA + dihydrolipoamide
-
 Oncorhynchus mykiss CoA + S-isovaleryldihydrolipoamide
-
 ?
additional information pyruvate is a poor substrate Oncorhynchus mykiss ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Oncorhynchus mykiss
More reconstitution of the multienzyme complex branched-chain alpha-keto dehydrogenase Oncorhynchus mykiss

Synonyms

Synonyms Comment Organism
More enzyme is the E2 component of the multienzyme complex branched-chain alpha-keto acid dehydrogenase, i.e. branched-chain 2-oxo acid dehydrogenase Oncorhynchus mykiss