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Literature summary for 2.3.1.168 extracted from
- Production of recombinant mammalian holo-E2 and E3 and reconstitution of functional branched-chain alpha-keto acid dehydrogenase complex with recombinant E1 (2000), Methods Enzymol., 324, 192-200.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of apo-enzyme in Escherichia coli strain XL1-Blue, can be lipoylated in vitro | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant apo-enzyme E2 from Escherichia coli strain XL1-Blue | Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine | the active site is located in the inner core domain | Bos taurus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant apo-E2 is unable to reconstitute with recombinant E1 and E3 to an active branched-chain alpha-keto dehydrogenase, but recombinant holo-E2 is able to | Bos taurus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.173 | - |
purified recombinant apo-enzyme | Bos taurus |
| 0.185 | - |
purified native enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine | recombinant apo-E2 devoid of lipoic acid is fully active | Bos taurus | CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | recombinant apo-E2 is unable to reconstitute with recombinant E1 and E3 to an active branched-chain alpha-keto dehydrogenase, but recombinant holo-E2 is able to | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme is the E2 component of the multienzyme complex branched-chain alpha-keto acid dehydrogenase, i.e. branched-chain 2-oxo acid dehydrogenase | Bos taurus |
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