Application | Comment | Organism |
---|---|---|
pharmacology | key enzyme in the biosynthesis of the anticancer drug paclitaxel, which catalyses the formation of baccatin III from 10-deacetylbaccatin III | Taxus mairei |
pharmacology | paclitaxel is a type of broad-spectrum anticancer drug in short supply. The price of acetyl-CoA, which is the acetyl group donor for the enzymatic synthesis of the intermediate, baccatin III, is the bottleneck of the mass production of paclitaxel. The study reports that N-acetyl-D-glucosamineas an acetyl group donor can substantially reduce the cost of production | Taxus mairei |
synthesis | key enzyme in the biosynthesis of the anticancer drug paclitaxel, which catalyses the formation of baccatin III from 10-deacetylbaccatin III | Taxus mairei |
synthesis | paclitaxel is a type of broad-spectrum anticancer drug in short supply. The price of acetyl-CoA, which is the acetyl group donor for the enzymatic synthesis of the intermediate, baccatin III, is the bottleneck of the mass production of paclitaxel. The study reports that N-acetyl-D-glucosamineas an acetyl group donor can substantially reduce the cost of production | Taxus mairei |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Taxus mairei |
Protein Variants | Comment | Organism |
---|---|---|
D166A | decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA | Taxus mairei |
D34E | decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA | Taxus mairei |
D372E | decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA | Taxus mairei |
G376A | decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA | Taxus mairei |
H162A | mutation results in complete loss of enzymatic activity, suggesting that the residue histidine at 162 is essential to DBAT activity | Taxus mairei |
R363A | decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA | Taxus mairei |
S31A | decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA | Taxus mairei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
0.073 | - |
acetyl-CoA | wild-type enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
0.075 | - |
10-deacetylbaccatin III | wild-type enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
0.134 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
0.148 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
0.231 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
0.29 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
0.762 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
0.925 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
1.094 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
1.51 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
1.67 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
2.28 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
3.21 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 10-deacetylbaccatin III | Taxus mairei | - |
CoA + baccatin III | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taxus mairei | H9BIH8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 10-deacetylbaccatin III | - |
Taxus mairei | CoA + baccatin III | - |
? | |
N-acetyl-D-glucosamine + 10-deacetylbaccatin III | the yield when using N-acetyl-D-glucosamine as acetyl donor in open-whole-cell catalytic system is 2.13fold of that when using acetyl-CoA. In the in vivo system, the yield increased 24.17%, which may indicate its cooperation with acetyl-CoA | Taxus mairei | D-glucosamine + baccatin III | - |
? | |
vinyl acetate + 10-deacetylbaccatin III | 42% of the activity as compared to N-acetyl-D-glucosamine | Taxus mairei | vinyl alcohol + baccatin III | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DBAT | - |
Taxus mairei |
taxoid 10beta-O-acetyl transferase | - |
Taxus mairei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.59 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
5.28 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
12.73 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
14.69 | - |
10-deacetylbaccatin III | wild-type enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
16.12 | - |
acetyl-CoA | wild-type enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
18.79 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
20.54 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
21.74 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
26.65 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
27.03 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
27.61 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
33.12 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
10 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
20 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
20 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
30 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
40 | - |
acetyl-CoA | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
70 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
120 | - |
10-deacetylbaccatin III | mutant enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
200 | - |
10-deacetylbaccatin III | wild-type enzyme, 30°C, pH not specified in the publication | Taxus mairei | |
220 | - |
acetyl-CoA | wild-type enzyme, 30°C, pH not specified in the publication | Taxus mairei |