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Literature summary for 2.3.1.167 extracted from

  • Walker.K.; Croteau, R.
    Molecular cloning of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli (2000), Proc. Natl. Acad. Sci. USA, 97, 583-587.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.167

Application

Application Comment Organism
pharmacology use in synthesis of taxol for anticnacer treatment Taxus cuspidata

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Taxus cuspidata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 acetyl-CoA
-
 Taxus cuspidata
0.01
-
 10-deacetylbaccatin III
-
 Taxus cuspidata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49050
-
 calculation from nucleotide sequence Taxus cuspidata
50000
-
 gel filtration Taxus cuspidata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 10-deacetylbaccatin III Taxus cuspidata
-
 CoA + baccatin III
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus cuspidata Q9M6E2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Taxus cuspidata

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Taxus cuspidata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 10-deacetylbaccatin III
-
 Taxus cuspidata CoA + baccatin III
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Taxus cuspidata

pH Range

pH Minimum pH Maximum Comment Organism
6.4 7.8
-
 Taxus cuspidata