Literature summary for 2.3.1.165 extracted from

Kallscheuer, N.; Kage, H.; Milke, L.; Nett, M.; Marienhagen, J.
Microbial synthesis of the type I polyketide 6-methylsalicylate with Corynebacterium glutamicum (2019), Appl. Microbiol. Biotechnol., 103, 9619-9631 .

Search for more literature for 2.3.1.165

Application

Application	Comment	Organism
synthesis	Corynebacterium glutamicum is used as a host for polyketide production. 6-Methylsalicylic acid synthase ChlB1 from Streptomyces antibioticus is introduced into this bacterium (ChlB1Sa). Challenges related to protein folding can be overcome by translation fusion of ChlB1Sa to the C-terminus of the maltose-binding protein MalE from Escherichia coli. ChlB1Sa is also active in the absence of a heterologous 4'-phosphopantetheinyl transferase, which leads to the discovery that the endogenous 4'-phosphopantetheinyl transferases PptACg of Corynebacterium glutamicum can also activate ChlB1Sa. 6-Methylsalicylic acid is an important building block in the biosynthesis of several antibiotics, including chlorothricin, maduropeptin, pactamycin, and polyketomycin	Streptomyces antibioticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + 3 malonyl-CoA + NADPH + H+	Streptomyces antibioticus	-	6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces antibioticus	Q0R4P8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + 3 malonyl-CoA + NADPH + H+	-	Streptomyces antibioticus	6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
ChlB1	-	Streptomyces antibioticus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)