Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.164 extracted from

  • Garcia-Estrada, C.; Vaca, I.; Fierro, F.; Sjollema, K.; Veenhuis, M.; Martin, J.F.
    The unprocessed preprotein form IATC103S of the isopenicillin N acyltransferase is transported inside peroxisomes and regulates its self-processing (2008), Fungal Genet. Biol., 45, 1043-1052.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
pPenIAT and pPenIATC103S are used to express the penDE gene in Penicillium chrysogenum, pPBCalphabeta, pDEC103S and palphabeta-DEC103S are used for expression in Escherichia coli Penicillium chrysogenum

Protein Variants

Protein Variants Comment Organism
C103S unprocessed inactive variant of isopenicillin N acyltransferase Penicillium chrysogenum

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Penicillium chrysogenum 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11000
-
processed protein, subunit alpha, determined by SDS-PAGE and Western blot analysis Penicillium chrysogenum
29000
-
processed protein, subunit beta, determined by SDS-PAGE and Western blot analysis Penicillium chrysogenum
40000
-
isopenicillin N acyltransferase precursor, proacyltransferase, proIAT, determined by SDS-PAGE and Western blot analysis Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetyl-CoA + isopenicillin N + H2O Penicillium chrysogenum
-
CoA + penicillin G + L-2-aminohexanedioate
-
?

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cell extracts of Penicillium chrysogenum are prepared, Escherichia coli soluble extracts are used for in vitro IAT activity measurement Penicillium chrysogenum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Penicillium chrysogenum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenylacetyl-CoA + isopenicillin N + H2O
-
Penicillium chrysogenum CoA + penicillin G + L-2-aminohexanedioate
-
?

Subunits

Subunits Comment Organism
heterodimer
-
Penicillium chrysogenum

Synonyms

Synonyms Comment Organism
IAT
-
Penicillium chrysogenum
isopenicillin N acyltransferase
-
Penicillium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
activity assay Penicillium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
activity assay Penicillium chrysogenum