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Literature summary for 2.3.1.164 extracted from

  • Tobin, M.B.; Cole, S.C.J.; Kovacevic, S.; Miller, J.R.; Baldwin, J.E.; Sutherland, J.D.
    Acyl-coenzyme A:isopenicillin N acyltransferase from Penicillium chrysogenum: effect of amino acid substitutions at Ser227, Ser230 and Ser309 on proenzyme cleavage and activity (1994), FEMS Microbiol. Lett., 121, 39-46.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 Penicillium chrysogenum

Protein Variants

Protein Variants Comment Organism
S227A produces uncleaved proenzyme lacking enzyme activity Penicillium chrysogenum
S309A recombinant proenzyme containing S309A is cleaved, however enzyme activity is not observed Penicillium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11000
-
alpha 1 * 11000 + beta 1 * 29000, wild-type and recombinant enzyme, SDS-PAGE Penicillium chrysogenum
29000
-
alpha 1 * 11000 + beta 1 * 29000, wild-type and recombinant enzyme, SDS-PAGE Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetyl-CoA + isopenicillin N + H2O Penicillium chrysogenum
-
CoA + benzylpenicillin + L-2-aminohexanedioate
-
?

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum
-
SC6140, ATCC 2044, source of native enzyme
-
Penicillium chrysogenum
-
penDE genes
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenylacetyl-CoA + 6-aminopenicillanic acid + H2O acyl-CoA:6-aminopenicillanate acyltransferase activity Penicillium chrysogenum CoA + benzylpenicillin
-
?
phenylacetyl-CoA + isopenicillin N + H2O
-
Penicillium chrysogenum CoA + benzylpenicillin + L-2-aminohexanedioate
-
?
phenylacetyl-CoA + isopenicillin N + H2O acyl-coenzyme A:isopenicillin N acyltransferase activity, S309 is involved in substrate acylation Penicillium chrysogenum CoA + benzylpenicillin + L-2-aminohexanedioate
-
?

Subunits

Subunits Comment Organism
heterodimer alpha 1 * 11000 + beta 1 * 29000, wild-type and recombinant enzyme, SDS-PAGE Penicillium chrysogenum