Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 | Penicillium chrysogenum |
Protein Variants | Comment | Organism |
---|---|---|
S227A | produces uncleaved proenzyme lacking enzyme activity | Penicillium chrysogenum |
S309A | recombinant proenzyme containing S309A is cleaved, however enzyme activity is not observed | Penicillium chrysogenum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11000 | - |
alpha 1 * 11000 + beta 1 * 29000, wild-type and recombinant enzyme, SDS-PAGE | Penicillium chrysogenum |
29000 | - |
alpha 1 * 11000 + beta 1 * 29000, wild-type and recombinant enzyme, SDS-PAGE | Penicillium chrysogenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetyl-CoA + isopenicillin N + H2O | Penicillium chrysogenum | - |
CoA + benzylpenicillin + L-2-aminohexanedioate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | - |
SC6140, ATCC 2044, source of native enzyme | - |
Penicillium chrysogenum | - |
penDE genes | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetyl-CoA + 6-aminopenicillanic acid + H2O | acyl-CoA:6-aminopenicillanate acyltransferase activity | Penicillium chrysogenum | CoA + benzylpenicillin | - |
? | |
phenylacetyl-CoA + isopenicillin N + H2O | - |
Penicillium chrysogenum | CoA + benzylpenicillin + L-2-aminohexanedioate | - |
? | |
phenylacetyl-CoA + isopenicillin N + H2O | acyl-coenzyme A:isopenicillin N acyltransferase activity, S309 is involved in substrate acylation | Penicillium chrysogenum | CoA + benzylpenicillin + L-2-aminohexanedioate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | alpha 1 * 11000 + beta 1 * 29000, wild-type and recombinant enzyme, SDS-PAGE | Penicillium chrysogenum |