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Literature summary for 2.3.1.162 extracted from

  • Walker, K.; Schoendorf, A.; Croteau, R.
    Molecular cloning of a taxa-4(20),11(12)-dien-5a-ol-O-acetyl transferase cDNA from taxus and functional expression in Escherichia coli (2000), Arch. Biochem. Biophys., 374, 371-380.
    View publication on PubMed
Search for more literature for 2.3.1.162

Application

Application Comment Organism
medicine understanding of biosynthetic pathway of taxol, target: possibly biological production of taxol, taxol or paclitaxel is now well established as a potent chemotherapeutic drug Taxus cuspidata
medicine understanding of biosynthetic pathway of taxol, target: possibly biological production of taxol, taxol or paclitaxel is now well established as a potent chemotherapeutic drug Taxus canadensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Taxus cuspidata
expression in Escherichia coli Taxus canadensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49080
-
 recombinant enzyme, calculated from amino acid composition Taxus canadensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Taxus cuspidata catalyzes the first acylation step of taxol biosynthesis ?
-
 ?
additional information Taxus canadensis catalyzes the first acylation step of taxol biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus canadensis
-
-
-
Taxus cuspidata
-
-
-
Taxus cuspidata Q9M6F0
-
-

Purification (Commentary)

Purification (Comment) Organism
800fold purification Taxus canadensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Taxus canadensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + taxa-4(20),11(12)-dien-5alpha-ol
-
 Taxus cuspidata CoA + taxa-4(20),11-dien-5alpha-yl acetate
-
 ?
acetyl-CoA + taxa-4(20),11(12)-dien-5alpha-ol
-
 Taxus canadensis CoA + taxa-4(20),11-dien-5alpha-yl acetate
-
 ?
additional information catalyzes the first acylation step of taxol biosynthesis Taxus cuspidata ?
-
 ?
additional information catalyzes the first acylation step of taxol biosynthesis Taxus canadensis ?
-
 ?