Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.160 extracted from

  • Ma, X.; Koepke, J.; Panjikar, S.; Fritzsch, G.; Stockigt, J.
    Crystal structure of vinorine synthase, the first representative of the BAHD superfamily (2005), J. Biol. Chem., 280, 13576-13583.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of N-terminally His-tagged enzyme in Escherichia coli Rauvolfia serpentina

Crystallization (Commentary)

Crystallization (Comment) Organism
purified detagged recombinant wild-type or selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 32°C, 2-3 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 mM acetyl-CoA, with reservoir solution containing 0.1 M Tris-HCl, pH 8.7, 2 M ammonium sulfate, 2% PEG 400, cryoprotection by 20-25% glycerol, X-ray diffraction structure determination anad analysis at 2.6 A resolution, modeling of CoA binding Rauvolfia serpentina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 16-epivellosimine Rauvolfia serpentina the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview CoA + vinorine
-
r

Organism

Organism UniProt Comment Textmining
Rauvolfia serpentina Q70PR7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration Rauvolfia serpentina

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + 16-epivellosimine = CoA + vinorine His160 of the HxxxD motif functions as a general base in catalysis, located at the center of the reaction channel at the interface of both domains and is accessible from both sides Rauvolfia serpentina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 16-epivellosimine
-
Rauvolfia serpentina CoA + vinorine
-
r
acetyl-CoA + 16-epivellosimine the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview Rauvolfia serpentina CoA + vinorine
-
r

Subunits

Subunits Comment Organism
monomer two-domain structure Rauvolfia serpentina

Synonyms

Synonyms Comment Organism
More enzyme belongs to the BAHD enzyme super family Rauvolfia serpentina
vinorine synthase
-
Rauvolfia serpentina
VS
-
Rauvolfia serpentina