Cloned (Comment) | Organism |
---|---|
overexpression of N-terminally His-tagged enzyme in Escherichia coli | Rauvolfia serpentina |
Crystallization (Comment) | Organism |
---|---|
purified detagged recombinant wild-type or selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 32°C, 2-3 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 mM acetyl-CoA, with reservoir solution containing 0.1 M Tris-HCl, pH 8.7, 2 M ammonium sulfate, 2% PEG 400, cryoprotection by 20-25% glycerol, X-ray diffraction structure determination anad analysis at 2.6 A resolution, modeling of CoA binding | Rauvolfia serpentina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 16-epivellosimine | Rauvolfia serpentina | the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview | CoA + vinorine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina | Q70PR7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration | Rauvolfia serpentina |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + 16-epivellosimine = CoA + vinorine | His160 of the HxxxD motif functions as a general base in catalysis, located at the center of the reaction channel at the interface of both domains and is accessible from both sides | Rauvolfia serpentina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 16-epivellosimine | - |
Rauvolfia serpentina | CoA + vinorine | - |
r | |
acetyl-CoA + 16-epivellosimine | the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview | Rauvolfia serpentina | CoA + vinorine | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | two-domain structure | Rauvolfia serpentina |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the BAHD enzyme super family | Rauvolfia serpentina |
vinorine synthase | - |
Rauvolfia serpentina |
VS | - |
Rauvolfia serpentina |