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Literature summary for 2.3.1.160 extracted from
- Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis (2004), Bioorg. Med. Chem., 12, 2781-2786.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0075 | - |
gardneral | pH 7.0, 30°C | Rauvolfia serpentina x Rhazya stricta |  |
| 0.057 | - |
acetyl-CoA | pH 7.0, 30°C | Rauvolfia serpentina x Rhazya stricta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
about, gel filtration | Rauvolfia serpentina x Rhazya stricta |
| 50000 | - |
1 * 50000, about, SDS-PAGE | Rauvolfia serpentina x Rhazya stricta |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + 16-epivellosimine | Rauvolfia serpentina x Rhazya stricta | the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview | CoA + vinorine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rauvolfia serpentina x Rhazya stricta | - |
hybrid | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 991fold from cell suspension culture by anion exchange, hydrophobic interaction, and hydroxyapatite chromatography, and gel filtration | Rauvolfia serpentina x Rhazya stricta |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | hybrid of Rauvolfia serpentina and Rhazya stricta | Rauvolfia serpentina x Rhazya stricta | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 99.1 | - |
purified enzyme | Rauvolfia serpentina x Rhazya stricta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + 16-epivellosimine | the enzyme is involved in a crucial step of antiarrhythmic drug ajmaline biosynthesis, pathway overview | Rauvolfia serpentina x Rhazya stricta | CoA + vinorine | - |
? | |
| acetyl-CoA + 16-epivellosimine | the forward reaction is preferred | Rauvolfia serpentina x Rhazya stricta | CoA + vinorine | - |
r | |
| acetyl-CoA + gardneral | sarpagan-type alkaloid, the forward reaction is preferred | Rauvolfia serpentina x Rhazya stricta | CoA + 11-methoxyvinorine | ajmalin-type alkaloid | r | |
| additional information | the acetylation is important for stabilization of the immediate indolenine 17-deacetyl-vinorine, which would otherwise be spontaneously re-transformed | Rauvolfia serpentina x Rhazya stricta | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50000, about, SDS-PAGE | Rauvolfia serpentina x Rhazya stricta |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | enzyme probably belongs to the BAHD enzyme super family | Rauvolfia serpentina x Rhazya stricta |
| vinorine synthase | - |
Rauvolfia serpentina x Rhazya stricta |
| VS | - |
Rauvolfia serpentina x Rhazya stricta |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rauvolfia serpentina x Rhazya stricta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Rauvolfia serpentina x Rhazya stricta |
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Release 2023.1 (January 2023)
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