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Literature summary for 2.3.1.16 extracted from

  • Srirangan, K.; Liu, X.; Tran, T.T.; Charles, T.C.; Moo-Young, M.; Chou, C.P.
    Engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using unrelated carbon sources (2016), Sci. Rep., 6, 36470 .
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. An Escherichia coli strain with an activated sleeping beauty mutase (Sbm) operon is used to generate propionyl-CoA as a precursor. Two acetyl-CoA moieties or acetyl-CoA and propionyl-CoA are condensed to form acetoacetyl-CoA and 3-ketovaleryl-CoA, respectively, by functional expression of beta-ketothiolases from Cupriavidus necator (i.e. PhaA and BktB). The resulting thioester intermediates are channeled into the polyhydroxyalkanoate biosynthetic pathway through functional expression of acetoacetyl-CoA reductase (PhaB) for thioester reduction and PHA synthase (PhaC) for subsequent polymerization. High-level poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production ranging from 3 to 19 mol%, can be achieved Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator Q0KBP1
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Cupriavidus necator ATCC 17699 Q0KBP1
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Synonyms

Synonyms Comment Organism
beta-ketothiolase
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 Cupriavidus necator
BktB
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 Cupriavidus necator