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Literature summary for 2.3.1.16 extracted from

  • Pantazaki, A.A.; Ioannou, A.K.; Kyriakidis, D.A.
    A thermostable beta-ketothiolase of polyhydroxyalkanoates (PHAs) in Thermus thermophilus: purification and biochemical properties (2005), Mol. Cell. Biochem., 269, 27-36.
    View publication on PubMed
Search for more literature for 2.3.1.16

Activating Compound

Activating Compound Comment Organism Structure
ATP activates Thermus thermophilus
NADH activates Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA substrate inhibition at high concentrations Thermus thermophilus
NEM inhibition at 1 mM in absence or presence of 0.5 mM acetyl-CoA Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Thermus thermophilus
0.011
-
 acetyl-CoA pH 8.0, 65°C Thermus thermophilus
0.025
-
 CoA pH 8.0, 65°C Thermus thermophilus
0.025
-
 acetoacetyl-CoA pH 8.0, 65°C Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Thermus thermophilus
Mg2+ activates Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45500
-
 4 * 45500, SDS-PAGE Thermus thermophilus
182000
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + H2O Thermus thermophilus
-
 acetate + CoA
-
 r
acetyl-CoA + H2O Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 acetate + CoA
-
 r
additional information Thermus thermophilus first enzyme of the most common biosynthetic pathway for polyhydroxyalkanoates, overview ?
-
 ?
additional information Thermus thermophilus HB8 / ATCC 27634 / DSM 579 first enzyme of the most common biosynthetic pathway for polyhydroxyalkanoates, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 386.4fold to homogeneity by anion exchange, hydrophobic interaction, and hydroxylapatite chromatography, and gel filtration Thermus thermophilus

Reaction

Reaction Comment Organism Reaction ID
acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA ping-pong mechanism, a cysteine residue in involved in the active site Thermus thermophilus
a

Renatured (Commentary)

Renatured (Comment) Organism
in situ renaturation of the enzyme from the SDS-polyacrylamide gel, after solubilization, SDS removal and denaturation with 6 M guanidine hydrochloride, overview Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1082
-
 purified enzyme Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O
-
 Thermus thermophilus acetate + CoA
-
 r
acetyl-CoA + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 acetate + CoA
-
 r
coenzyme A + acetoacetyl-CoA
-
 Thermus thermophilus 2 acetyl-CoA
-
 ?
coenzyme A + acetoacetyl-CoA
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 2 acetyl-CoA
-
 ?
additional information first enzyme of the most common biosynthetic pathway for polyhydroxyalkanoates, overview Thermus thermophilus ?
-
 ?
additional information first enzyme of the most common biosynthetic pathway for polyhydroxyalkanoates, overview Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 45500, SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
beta-ketothiolase
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 for both reaction directions Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 15 min, purified enzyme, completely stable Thermus thermophilus
100
-
 15 min, purified enzyme, loss of 23% of activity Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 for both reaction directions Thermus thermophilus