Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli | Ruta graveolens |
Protein Variants | Comment | Organism |
---|---|---|
S132T/A133S/V265F | site-directed mutagenesis, exchange of 3 residues, responsible for substrate activity, by the corresponding amino acids of chalcone synthase results in transformation of the enzyme to a chalcone synthase, 25fold increased activity, with only 36% remaining acridone synthase activity compared to wild-type | Ruta graveolens |
V265F | site-directed mutagenesis, 75% reduction of ACS catalytic activity, but 2fold increased chalcone synthase activity | Ruta graveolens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
structure model of isozyme II | Ruta graveolens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + N-methylanthraniloyl-CoA | Ruta graveolens | - |
CoA + 1,3-dihydroxy-N-methylacridone + CO2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruta graveolens | - |
2 isozymes I and II | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3 malonyl-CoA + N-methylanthraniloyl-CoA = 4 CoA + 1,3-dihydroxy-N-methylacridone + 3 CO2 + H2O | two-step mechanism | Ruta graveolens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + 4-coumaroyl-CoA | wild-type shows low activity, while mutants show highly increased activity | Ruta graveolens | CoA + naringenin chalcone | - |
? | |
malonyl-CoA + N-methylanthraniloyl-CoA | - |
Ruta graveolens | CoA + 1,3-dihydroxy-N-methylacridone + CO2 | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
ACS | - |
Ruta graveolens |