Literature summary for 2.3.1.159 extracted from

Springob, K.; Lukacin, R.; Ernwein, C.; Groning, I.; Matern, U.
Specificities of functionally expressed chalcone and acridone synthases from Ruta graveolens (2000), Eur. J. Biochem., 267, 6552-6559.

Search for more literature for 2.3.1.159

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21 (DE3)pLys S and in lambda phages via Escherichia coli host, DNA sequence comparison of 4 clones and also with the DNA sequence of Ruta graveolens chalcone synthase	Ruta graveolens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
malonyl-CoA + N-methylanthraniloyl-CoA	Ruta graveolens	-	CoA + 1,3-dihydroxy-N-methylacridone + CO2	-	ir

Organism

Organism	UniProt	Comment	Textmining
Ruta graveolens	-	2 isozymes I and II	-

Reaction

Reaction	Comment	Organism	Reaction ID
3 malonyl-CoA + N-methylanthraniloyl-CoA = 4 CoA + 1,3-dihydroxy-N-methylacridone + 3 CO2 + H2O	two-step mechanism	Ruta graveolens	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell suspension culture	-	Ruta graveolens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Ruta graveolens
9.54	-	purified recombinant isozyme I	Ruta graveolens
14.16	-	purified recombinant isozyme II	Ruta graveolens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
malonyl-CoA + 4-coumaroyl-CoA	isozyme I shows 16% activity and isozyme II shows 12% activity compared to the activity with N-methylanthraniloyl-CoA as substrate, respectively	Ruta graveolens	CoA + naringenin chalcone	-	?
malonyl-CoA + N-methylanthraniloyl-CoA	-	Ruta graveolens	CoA + 1,3-dihydroxy-N-methylacridone + CO2	-	ir

Synonyms

Synonyms	Comment	Organism
ACS	-	Ruta graveolens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)