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Literature summary for 2.3.1.158 extracted from

  • Zhang, H.; Damude, H.G.; Yadav, N.S.
    Three diacylglycerol acyltransferases contribute to oil biosynthesis and normal growth in Yarrowia lipolytica (2012), Yeast, 29, 25-38.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pdat or YALI0E16797g, DNA and amino acid sequence determination and analysis. In vivo expression of DGAT1, DGAT2 and PDAT under the heterologous Yarrowia lipolytica YAT promoter results in 598%, 702% and 278% increases in total fatty acid % dry cell weight over the empty vector control Yarrowia lipolytica

Protein Variants

Protein Variants Comment Organism
additional information construction of pdat, diacylglycerol O-acyltransferases (EC 2.3.1.20) dgat1 and dgat2 single mutants, of dgat1/dgat2 double mutant, and of a dgat1/dgat2/pdat triple mutant, which show 70%, 57%, 36%, 18% and 13%, respectively, of wild-type total lipid% per dry cell weight, phenotypes, overview Yarrowia lipolytica

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica Q6C5M4 gene pdat
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Yarrowia lipolytica ATCC 20362 Q6C5M4 gene pdat
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Synonyms

Synonyms Comment Organism
acyl-CoA-independent phospholipid:diacylglycerol acyltransferase
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Yarrowia lipolytica
PDAT
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Yarrowia lipolytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
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assay at Yarrowia lipolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
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assay at Yarrowia lipolytica