Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
C380A | the acetyltransferase activity of the mutant is 2.6fold higher than that of the wild type enzyme | Bacillus subtilis |
C380A/C405S | the double mutant has an increased kcat/Km value compared to the wild type enzyme | Bacillus subtilis |
C405A | the mutant shows a slight increase in activity compared to the wild type enzyme | Bacillus subtilis |
C405S | the mutant has an increased kcat/Km value compared to the wild type enzyme | Bacillus subtilis |
V385C/C405S | the double mutant has an increased kcat/Km value compared to the wild type enzyme | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.199 | - |
acetyl-CoA | mutant enzyme V385C/C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
0.241 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme C380A, at pH 8.0 and 37°C | Bacillus subtilis | |
0.278 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme C405A, at pH 8.0 and 37°C | Bacillus subtilis | |
0.304 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme V385C/C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
0.333 | - |
alpha-D-glucosamine 1-phosphate | wild type enzyme, at pH 8.0 and 37°C | Bacillus subtilis | |
0.359 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
0.47 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme C380A/C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
0.603 | - |
acetyl-CoA | mutant enzyme C380A, at pH 8.0 and 37°C | Bacillus subtilis | |
0.638 | - |
acetyl-CoA | mutant enzyme C380A/C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
1.213 | - |
acetyl-CoA | mutant enzyme C405A, at pH 8.0 and 37°C | Bacillus subtilis | |
1.821 | - |
acetyl-CoA | wild type enzyme, at pH 8.0 and 37°C | Bacillus subtilis | |
1.963 | - |
acetyl-CoA | mutant enzyme C405S, at pH 8.0 and 37°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the concentration of Mg2+ has no effect on the enzymatic activity | Escherichia coli | |
Mg2+ | the enzyme activity is optimal at 15 mM | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + alpha-D-glucosamine 1-phosphate | Escherichia coli | - |
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | Bacillus subtilis | - |
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | Bacillus subtilis 168 | - |
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P14192 | - |
- |
Bacillus subtilis 168 | P14192 | - |
- |
Escherichia coli | P0ACC7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA His-binding column chromatography | Escherichia coli |
Ni-NTA His-binding column chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Escherichia coli | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Bacillus subtilis | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Bacillus subtilis 168 | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, SDS-PAGE | Escherichia coli |
? | x * 52000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
GlmU | bifunctional enzyme with glucosamine 1-phosphate acetyltransferase and N-acetylglucosamine 1-phosphate uridyltransferase activities | Escherichia coli |
GlmU | bifunctional enzyme with glucosamine 1-phosphate acetyltransferase and N-acetylglucosamine 1-phosphate uridyltransferase activities | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Escherichia coli |
37 | - |
- |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.81 | - |
alpha-D-glucosamine 1-phosphate | wild type enzyme, at pH 8.0 and 37°C | Bacillus subtilis | |
2.407 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
2.459 | - |
acetyl-CoA | wild type enzyme, at pH 8.0 and 37°C | Bacillus subtilis | |
3.112 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme C405A, at pH 8.0 and 37°C | Bacillus subtilis | |
5.496 | - |
acetyl-CoA | mutant enzyme C405A, at pH 8.0 and 37°C | Bacillus subtilis | |
6.629 | - |
acetyl-CoA | mutant enzyme V385C/C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
15.756 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme V385C/C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
18.512 | - |
acetyl-CoA | mutant enzyme C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
25.441 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme C380A, at pH 8.0 and 37°C | Bacillus subtilis | |
26.147 | - |
acetyl-CoA | mutant enzyme C380A/C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
30.783 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme C380A/C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
54.617 | - |
acetyl-CoA | mutant enzyme C380A, at pH 8.0 and 37°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |
8 | - |
- |
Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.35 | - |
acetyl-CoA | wild type enzyme, at pH 8.0 and 37°C | Bacillus subtilis | |
2.432 | - |
alpha-D-glucosamine 1-phosphate | wild type enzyme, at pH 8.0 and 37°C | Bacillus subtilis | |
4.531 | - |
acetyl-CoA | mutant enzyme C405A, at pH 8.0 and 37°C | Bacillus subtilis | |
6.705 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
9.43 | - |
acetyl-CoA | mutant enzyme C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
11.194 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme C405A, at pH 8.0 and 37°C | Bacillus subtilis | |
33.262 | - |
acetyl-CoA | mutant enzyme V385C/C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
40.977 | - |
acetyl-CoA | mutant enzyme C380A/C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
51.829 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme V385C/C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
65.496 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme C380A/C405S, at pH 8.0 and 37°C | Bacillus subtilis | |
90.546 | - |
acetyl-CoA | mutant enzyme C380A, at pH 8.0 and 37°C | Bacillus subtilis | |
105.564 | - |
alpha-D-glucosamine 1-phosphate | mutant enzyme C380A, at pH 8.0 and 37°C | Bacillus subtilis |