Crystallization (Comment) | Organism |
---|---|
apo GlmU is crystallized at 20 °C using the sitting-drop vapor diffusion methodcrystal structure of a mimic of the Michaelis complex, with glucose 1-phosphate and acetyl-coenzyme A, helps us to propose the residues involved in deprotonation of glucosamine 1-phosphate and the loop movement that likely generates the active site required for glucosamine 1-phosphate to bind | Mycobacterium tuberculosis |
apo GlmU is crystallized at 20 °C using the sitting-drop vapor diffusion methodcrystal structure of a mimic of the Michaelis complex, with glucose 1-phosphate and acetyl-coenzyme A, indicates the residues involved in deprotonation of glucosamine 1-phosphate and the loop movement that likely generates the active site required for glucosamine 1-phosphate to bind | Mycobacterium tuberculosis |
in complex with acetyl-CoA and glucose 1-phosphate | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
butanoyl-CoA | - |
Mycobacterium tuberculosis | |
CoA | - |
Mycobacterium tuberculosis | |
crotonyl-CoA | - |
Mycobacterium tuberculosis | |
D-galactosamine 1-phosphate | - |
Mycobacterium tuberculosis | |
D-glucosamine 6-phosphate | - |
Mycobacterium tuberculosis | |
D-glucose 1-phosphate | - |
Mycobacterium tuberculosis | |
ethyl-CoA | - |
Mycobacterium tuberculosis | |
isobutanoyl-CoA | - |
Mycobacterium tuberculosis | |
malonyl-CoA | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
alpha-D-glucosamine 1-phosphate | cosubstrate acetoacetyl-CoA, pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.016 | - |
alpha-D-glucosamine 1-phosphate | pH 7.5, 30°C, cosubstrate: acetoacetyl-CoA | Mycobacterium tuberculosis | |
0.06 | - |
propionyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.06 | - |
n-propionyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.25 | - |
acetyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.29 | - |
alpha-D-glucosamine 1-phosphate | pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.29 | - |
alpha-D-glucosamine 1-phosphate | cosubstrate acetyl-CoA, pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.98 | - |
alpha-D-glucosamine 1-phosphate | cosubstrate propionyl-CoA, pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.98 | - |
alpha-D-glucosamine 1-phosphate | pH 7.5, 30°C, cosubstrate: n-propionyl-CoA | Mycobacterium tuberculosis | |
1.1 | - |
succinyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
1.8 | - |
acetoacetyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Mycobacterium tuberculosis | |
Mg2+ | required | Mycobacterium tuberculosis | |
Mn2+ | activates | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + alpha-D-glucosamine 1-phosphate | Mycobacterium tuberculosis | - |
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | Mycobacterium tuberculosis | the enzyme is responsible for the final two steps of the synthesis of UDP-N-acetylglucosamine, which is an essential precursor of peptidoglycan | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | Mycobacterium tuberculosis ATCC 25177 | - |
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | Mycobacterium tuberculosis ATCC 25177 | the enzyme is responsible for the final two steps of the synthesis of UDP-N-acetylglucosamine, which is an essential precursor of peptidoglycan | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | A5U161 | - |
- |
Mycobacterium tuberculosis | A5U161 | bifunctional N-acetyltransferase/uridylyltransferase, catalyzes reactions of EC 2.3.1.157 and EC 2.7.7.23 | - |
Mycobacterium tuberculosis ATCC 25177 | A5U161 | - |
- |
Mycobacterium tuberculosis ATCC 25177 | A5U161 | bifunctional N-acetyltransferase/uridylyltransferase, catalyzes reactions of EC 2.3.1.157 and EC 2.7.7.23 | - |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Mycobacterium tuberculosis | CoA + N-acetoacetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetoacetyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Mycobacterium tuberculosis ATCC 25177 | CoA + N-acetoacetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Mycobacterium tuberculosis | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | the enzyme is responsible for the final two steps of the synthesis of UDP-N-acetylglucosamine, which is an essential precursor of peptidoglycan | Mycobacterium tuberculosis | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | the enzyme utilizes ternary complex formation to transfer an acetyl from acetyl-coenzyme A to glucosamine 1-phosphate to form N-acetylglucosamine 1-phosphate. Steady-state kinetic studies and equilibrium binding experiments indicate that GlmU follows a steady-state ordered kinetic mechanism, with acetyl-coenzyme A binding first, which triggers a conformational change in GlmU, followed by glucosamine 1-phosphate binding. Coenzyme A is the last product to dissociate | Mycobacterium tuberculosis | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Mycobacterium tuberculosis ATCC 25177 | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | the enzyme is responsible for the final two steps of the synthesis of UDP-N-acetylglucosamine, which is an essential precursor of peptidoglycan | Mycobacterium tuberculosis ATCC 25177 | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
acetyl-CoA + alpha-D-glucosamine 1-phosphate | the enzyme utilizes ternary complex formation to transfer an acetyl from acetyl-coenzyme A to glucosamine 1-phosphate to form N-acetylglucosamine 1-phosphate. Steady-state kinetic studies and equilibrium binding experiments indicate that GlmU follows a steady-state ordered kinetic mechanism, with acetyl-coenzyme A binding first, which triggers a conformational change in GlmU, followed by glucosamine 1-phosphate binding. Coenzyme A is the last product to dissociate | Mycobacterium tuberculosis ATCC 25177 | CoA + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
additional information | GlmU follows a steady-state ordered kinetic mechanism, with acetyl-coenzyme A binding first, which triggers a conformational change in GlmU, followed by glucosamine 1-phosphate binding. Coenzyme A is the last product to dissociate | Mycobacterium tuberculosis | ? | - |
- |
|
additional information | GlmU follows a steady-state ordered kinetic mechanism, with acetyl-coenzyme A binding first, which triggers a conformational change in GlmU, followed by glucosamine 1-phosphate binding. Coenzyme A is the last product to dissociate | Mycobacterium tuberculosis ATCC 25177 | ? | - |
- |
|
n-propionyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Mycobacterium tuberculosis | CoA + N-propionyl-alpha-D-glucosamine 1-phosphate | - |
? | |
propionyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Mycobacterium tuberculosis | CoA + N-propionyl-alpha-D-glucosamine 1-phosphate | - |
? | |
succinyl-CoA + alpha-D-glucosamine 1-phosphate | - |
Mycobacterium tuberculosis | CoA + N-succinyl-alpha-D-glucosamine 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bifunctional N-acetyltransferase/uridylyltransferase | - |
Mycobacterium tuberculosis |
GlmU | - |
Mycobacterium tuberculosis |
GlmU | bifunctional enzyme, also to possess the activity of EC 2.3.1.23, glucosamine-1-phosphate N-acetyltransferase | Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
alpha-D-glucosamine 1-phosphate | cosubstrate acetoacetyl-CoA, pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.13 | - |
alpha-D-glucosamine 1-phosphate | pH 7.5, 30°C, cosubstrate: acetoacetyl-CoA | Mycobacterium tuberculosis | |
0.5 | - |
acetoacetyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.5 | - |
propionyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.5 | - |
n-propionyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.5 | - |
alpha-D-glucosamine 1-phosphate | cosubstrate propionyl-CoA, pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.5 | - |
alpha-D-glucosamine 1-phosphate | pH 7.5, 30°C, cosubstrate: n-propionyl-CoA | Mycobacterium tuberculosis | |
0.9 | - |
succinyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
2 | 8 | acetyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
2 | 8 | alpha-D-glucosamine 1-phosphate | pH 7.5, 30°C | Mycobacterium tuberculosis | |
2 | 8 | alpha-D-glucosamine 1-phosphate | cosubstrate acetyl-CoA, pH 7.5, 30°C | Mycobacterium tuberculosis | |
2 | 8 | alpha-D-glucosamine 1-phosphate | pH 7.5, 30°C, cosubstrate: acetyl-CoA | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.3 | - |
pH 7.5, 30°C | Mycobacterium tuberculosis | CoA | |
1 | - |
pH 7.5, 30°C | Mycobacterium tuberculosis | crotonyl-CoA | |
1 | - |
pH 7.5, 30°C | Mycobacterium tuberculosis | butanoyl-CoA | |
2 | - |
pH 7.5, 30°C | Mycobacterium tuberculosis | malonyl-CoA | |
2 | - |
pH 7.5, 30°C | Mycobacterium tuberculosis | isobutanoyl-CoA | |
5 | - |
pH 7.5, 30°C | Mycobacterium tuberculosis | D-glucose 1-phosphate | |
10 | - |
pH 7.5, 30°C | Mycobacterium tuberculosis | D-glucosamine 6-phosphate | |
120 | - |
pH 7.5, 30°C | Mycobacterium tuberculosis | D-galactosamine 1-phosphate |
General Information | Comment | Organism |
---|---|---|
metabolism | the bifunctional enzyme is responsible for the final two steps of the synthesis of UDP-N-acetylglucosamine, which is an essential precursor of peptidoglycan | Mycobacterium tuberculosis |
metabolism | the enzyme is responsible for the final two steps of the synthesis of UDP-N-acetylglucosamine, which is an essential precursor of peptidoglycan | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
acetoacetyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.51 | - |
alpha-D-glucosamine 1-phosphate | cosubstrate propionyl-CoA, pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.51 | - |
alpha-D-glucosamine 1-phosphate | pH 7.5, 30°C, cosubstrate: n-propionyl-CoA | Mycobacterium tuberculosis | |
0.82 | - |
succinyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
8.1 | - |
alpha-D-glucosamine 1-phosphate | cosubstrate acetoacetyl-CoA, pH 7.5, 30°C | Mycobacterium tuberculosis | |
8.1 | - |
alpha-D-glucosamine 1-phosphate | pH 7.5, 30°C, cosubstrate: acetoacetyl-CoA | Mycobacterium tuberculosis | |
8.3 | - |
propionyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
8.3 | - |
n-propionyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
97 | - |
alpha-D-glucosamine 1-phosphate | pH 7.5, 30°C | Mycobacterium tuberculosis | |
97 | - |
alpha-D-glucosamine 1-phosphate | cosubstrate acetyl-CoA, pH 7.5, 30°C | Mycobacterium tuberculosis | |
110 | - |
acetyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis | |
112 | - |
acetyl-CoA | pH 7.5, 30°C | Mycobacterium tuberculosis |