Application | Comment | Organism |
---|---|---|
nutrition | cones of the hop plant used in the beer-brewing process | Humulus lupulus |
Cloned (Comment) | Organism |
---|---|
characterization of the VPS cDNA clone | Humulus lupulus |
General Stability | Organism |
---|---|
low stability | Humulus lupulus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
isovaleryl-CoA | - |
Humulus lupulus | |
0.01 | - |
isobutyryl-CoA | - |
Humulus lupulus | |
0.033 | - |
malonyl-CoA | - |
Humulus lupulus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
homodimer, 2 * 45000, SDS-PAGE | Humulus lupulus |
110000 | - |
native PAGE of purified VPS | Humulus lupulus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Humulus lupulus | - |
hop | - |
Purification (Comment) | Organism |
---|---|
- |
Humulus lupulus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
isovaleryl-CoA + 3 malonyl-CoA = 4 CoA + 3 CO2 + phlorisovalerophenone | Closely related to EC 2.3.1.74, chalcone synthase. The product, 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one, is chloroisovalerophenone. Also acts on isobutyryl-CoA as substrate to give phlorisobutyrophenone. The products are intermediates in the biosynthesis of the bitter alpha-acids in hops, Humulus lupulus | Humulus lupulus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cone | glandular hairs | Humulus lupulus | - |
inflorescence | glands | Humulus lupulus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isobutyryl-CoA + 3 malonyl-CoA | - |
Humulus lupulus | phloroisobutyrophenone + 3 CO2 + 4 CoA | - |
? | |
isovaleryl-CoA + malonyl-CoA | - |
Humulus lupulus | phloroisovalerophenone + CO2 + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer, 2 * 45000, SDS-PAGE | Humulus lupulus |
Synonyms | Comment | Organism |
---|---|---|
VPS | - |
Humulus lupulus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Humulus lupulus |