Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PCMB | 90% inhibition at 0.1 mM, complete inhibition at 1 mM | Gentiana triflora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
caffeoyl-CoA | with delphinidin 3,5-diglucoside as cosubstrate | Gentiana triflora | |
0.087 | - |
cyanidin 3,5-diglucoside | with caffeoyl-CoA as cosubstrate | Gentiana triflora | |
0.15 | - |
delphinidin 3,5-diglucoside | with caffeoyl-CoA as cosubstrate | Gentiana triflora | |
0.19 | - |
p-Coumaroyl-CoA | with delphinidin 3,5-diglucoside as cosubstrate | Gentiana triflora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | strongly enhances activity | Gentiana triflora | |
Zn2+ | moderately enhances activity | Gentiana triflora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
gel filtration | Gentiana triflora |
52000 | - |
1 * 52000, SDS-PAGE | Gentiana triflora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gentiana triflora | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gentiana triflora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
petal | blue | Gentiana triflora | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Gentiana triflora |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris/HCl, pH 7.0, 0.01 mM (p-aminidinophenyl)methanesulfonyl fluoride, 1 mM DTT, stable for at least 2 years | Gentiana triflora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyanidin-3,5-O-beta-D-diglucoside + 4-coumaroyl-CoA | - |
Gentiana triflora | CoA + cyanidin 3-O-beta-D-glucoside-5-O-(6-O-4-coumaroyl)-beta-D-glucoside | - |
? | |
cyanidin-3,5-O-beta-D-diglucoside + caffeoyl-CoA | - |
Gentiana triflora | CoA + cyanidin 3-O-beta-D-glucoside-5-O-(6-O-caffeoyl)-beta-D-glucoside | - |
? | |
delphinidin 3,5-di-O-beta-D-glucoside + 4-coumaroyl-CoA | - |
Gentiana triflora | CoA + delphinidin 3-O-beta-D-glucoside-5-O-(6-O-4-coumaroyl)-beta-D-glucoside | - |
? | |
delphinidin 3,5-di-O-beta-D-glucoside + caffeoyl-CoA | - |
Gentiana triflora | CoA + delphinidin 3-O-beta-D-glucosyl-5-O-(6-O-caffeoyl-beta-D-glucoside) | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, SDS-PAGE | Gentiana triflora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Gentiana triflora |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | active in this range | Gentiana triflora |