Cloned (Comment) | Organism |
---|---|
gene plat2, DNA and amino acid sequence determination and analysis, the sequence of plat2 from Aurantiochytrium limacinum strain ATCC MYA-1381 is used for screening, sequence comparisons and phylogenetic tree, recombinant overexpression in Saccharoymces cerevisiae strain INVSc1, functional recombinant overexpression of FLAG-tagged enzyme in Aurantiochytrium limacinum strain F26-b, subcloning in Escherichia coli strain JM109 | Aurantiochytrium limacinum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruption and overexpression of the PLAT2 in Aurantiochytrium limacinum leading to reduced and increased enzyme activity, i.e. plat2-disruption mutant (plat2-KO) and plat2-overexpression mutant (plat2-OE), respectively, overview. The plat2 KO vector, which contains the promoter, hygromycin B phosphotransferase gene (hygromycin resistance gene, HygR), and terminator, i.e. HygR cassette | Aurantiochytrium limacinum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme sequence contains three possible transmembrane domains | Aurantiochytrium limacinum | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Aurantiochytrium limacinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Aurantiochytrium limacinum | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | Aurantiochytrium limacinum F26-b | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
docosahexaenoyl-CoA + sn-glycerol 3-phosphate | Aurantiochytrium limacinum | - |
CoA + 1-docosahexaenoyl-sn-glycerol 3-phosphate | - |
? | |
docosahexaenoyl-CoA + sn-glycerol 3-phosphate | Aurantiochytrium limacinum F26-b | - |
CoA + 1-docosahexaenoyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aurantiochytrium limacinum | A0A387K406 | isolated from fallen leaves of Rhizophora mucronata collected at Ishigaki Is., Okinawa, Japan | - |
Aurantiochytrium limacinum F26-b | A0A387K406 | isolated from fallen leaves of Rhizophora mucronata collected at Ishigaki Is., Okinawa, Japan | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Aurantiochytrium limacinum | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | - |
Aurantiochytrium limacinum F26-b | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
docosahexaenoyl-CoA + sn-glycerol 3-phosphate | - |
Aurantiochytrium limacinum | CoA + 1-docosahexaenoyl-sn-glycerol 3-phosphate | - |
? | |
docosahexaenoyl-CoA + sn-glycerol 3-phosphate | - |
Aurantiochytrium limacinum F26-b | CoA + 1-docosahexaenoyl-sn-glycerol 3-phosphate | - |
? | |
additional information | PLAT2 is a GPAT that transfers DHA to G3P in vivo as well as in vitro, substrate specificity, detailed overview | Aurantiochytrium limacinum | ? | - |
- |
|
additional information | PLAT2 is a GPAT that transfers DHA to G3P in vivo as well as in vitro, substrate specificity, detailed overview | Aurantiochytrium limacinum F26-b | ? | - |
- |
|
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Aurantiochytrium limacinum | CoA + palmitoyl-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Aurantiochytrium limacinum F26-b | CoA + palmitoyl-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 77200, about, sequence calculation | Aurantiochytrium limacinum |
Synonyms | Comment | Organism |
---|---|---|
G3P acyltransferase | - |
Aurantiochytrium limacinum |
glycerol-3-phosphate acyltransferase | - |
Aurantiochytrium limacinum |
GPAT | - |
Aurantiochytrium limacinum |
PLAT2 | - |
Aurantiochytrium limacinum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Aurantiochytrium limacinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Aurantiochytrium limacinum |
General Information | Comment | Organism |
---|---|---|
evolution | PLAT2 is highly homologous to the GPAT isozymes 3 and 4 | Aurantiochytrium limacinum |
malfunction | GPAT activity is reduced by disruption of the PLAT2 gene in Aurantiochytrium limacinum, resulting in a decrease in DHA-containing lysophosphatidic acid (LPA 22:6). Conversely, overexpression of PLAT2 increases both GPAT activity and LPA 22:6. Overexpression of the PLAT2 gene increases the production of a two DHA-containing diacylglycerol (DG 44:12), followed by an increase in the three DHA-containing triacylglycerol (TG 66:18), two-DHA-containing TG (TG 60:12), and two DHA-containing phosphatidylcholine (PC 44:12). Overexpression of PLAT2 does not increase DHA-free DG (DG32:0), which is preferentially converted to three 16:0-containing TG (TG 48:0) but not two 16:0-containing PC (PC 32:0) | Aurantiochytrium limacinum |
metabolism | PLAT2 is a rate-limiting enzyme in docosahexaenoic acid (DHA)-rich glycerolipid synthesis in Aurantiochytrium limacinum | Aurantiochytrium limacinum |
additional information | the enzyme sequence contains four acyltransferase motifs, sequence comparisons, overview | Aurantiochytrium limacinum |
physiological function | the glycerol-3-phosphate acyltransferase PLAT2 functions in the generation of docosahexaenoic acid (DHA)-rich glycerolipids in Aurantiochytrium limacinum strain F26-b. DHA-rich glycerolipids are produced from DHA-containing lysophosphatidic acid (LPA 22:6) as a precursor, which is generated by incorporating DHA to glycerol-3-phosphate (G3P) by phospholipid acyltransferase 2 (PLAT2) | Aurantiochytrium limacinum |