Cloned (Comment) | Organism |
---|---|
gene plsB1 or Rv1551, DNA and amino acid sequence determination and analysis, sequence comparisons, subcloning in Escherichia coli strain TOP10, functional recombinant expression in Escherichia coli strain BL21 Star (DE3) leading to significantly (2-4fold) higher viable cell counts during the exponential and stationary phases. Growth of Escherichia coli in medium containing long-chain fatty acids is enhanced by mGPAT. The enzyme displays function as a GPAT by enhancing the synthesis of phospholipids from exogenously provided fatty acids in Escherichia coli cell lysates | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Mycobacterium tuberculosis | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | Mycobacterium tuberculosis H37Rv | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | Mycobacterium tuberculosis ATCC 25618 | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WI59 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WI59 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WI59 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Mycobacterium tuberculosis | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | - |
Mycobacterium tuberculosis H37Rv | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | - |
Mycobacterium tuberculosis ATCC 25618 | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 69000, recombinant detagged enzyme, SDS-PAGE, x * 72000, recombinant tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-phosphate acyltransferase | - |
Mycobacterium tuberculosis |
GPAT | - |
Mycobacterium tuberculosis |
mGPAT | - |
Mycobacterium tuberculosis |
PlsB1 | - |
Mycobacterium tuberculosis |
Rv1551 | - |
Mycobacterium tuberculosis |
Rv1551 acyltransferase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the initial step of glycerophospholipid biosynthesis in the mycobacterial cell, pathway overview | Mycobacterium tuberculosis |
physiological function | glycerol-3-phosphate acyltransferase (GPAT) catalyzes the first step of the glycerophospholipid biosynthetic pathway that synthesizes the lipid precursors for triacylglycerol biosynthesis. GPAT catalyzes the acylation of glycerol-3-phosphate to form lysophosphatidic acid. Role of GPAT in dormancy-associated triacylglycerol synthesis in Mycobacterium tuberculosis | Mycobacterium tuberculosis |