Literature summary for 2.3.1.15 extracted from

Men, X.; Shi, J.; Liang, W.; Zhang, Q.; Lian, G.; Quan, S.; Zhu, L.; Luo, Z.; Chen, M.; Zhang, D.
Glycerol-3-phosphate acyltransferase 3 (OsGPAT3) is required for anther development and male fertility in rice (2017), J. Exp. Bot., 68, 513-526 .

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determmination and analysis, phylogenetic analysis and tree, quantitative reverse-transcription real-time PCR expression analysis, functional complementation of the osgpat3 mutant, calluses induced from young panicles of the homozygous osgpat3 plants are used for transformation with Agrobacterium tumefaciens strain EHA105 carrying the pCAMBIA1301-OsGPAT3-GFP plasmid or the control plasmid pCAMBIA1301-GFP expressing GFP-tagged wild-type and mutant enzymes, map-based cloning and functional complementation of mutant osgpat3, localization of osgpat3 mutation on chromosome 11	Oryza sativa

Cloned (Comment)

Organism

DNA and amino acid sequence determmination and analysis, phylogenetic analysis and tree, quantitative reverse-transcription real-time PCR expression analysis, functional complementation of the osgpat3 mutant, calluses induced from young panicles of the homozygous osgpat3 plants are used for transformation with Agrobacterium tumefaciens strain EHA105 carrying the pCAMBIA1301-OsGPAT3-GFP plasmid or the control plasmid pCAMBIA1301-GFP expressing GFP-tagged wild-type and mutant enzymes, map-based cloning and functional complementation of mutant osgpat3, localization of osgpat3 mutation on chromosome 11

Oryza sativa

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of an enzyme-deficient gpat3 mutant, the mutant exhibits normal vegetative development and inflorescence morphology, but has pale yellow to white and much smaller anthers compared with those of the wild-type and lacks mature pollen grains at the late stages of anther development, phenotype, detailed overview. Map-based cloning and functional complementation of mutant osgpat3, localization of osgpat3 mutation on chromosome 11	Oryza sativa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	the N-terminus of OsGPAT3 is not a signal peptide	Oryza sativa	5783	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acyl-CoA + sn-glycerol 3-phosphate	Oryza sativa	-	CoA + 1-acyl-sn-glycerol 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	Q2QZN3	subspecies japonica and indica are crossed	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
anther	OsGPAT3 is preferentially expressed in the tapetum and microspores of the anther. In anther tissues, the expression of OsGPAT3 is detectable as early as stage 7, peaks at stage 8a, declined gradually until stage 10, then increases and peaks again at a lower level at stage 12, and thereafter declines, dynamic expression pattern of OsGPAT3	Oryza sativa	-
additional information	the F2 mapping population is generated from a cross between osgpat3 (japonica) and GuangLuAi 4 (wild-type, indica) for gene mapping. Male sterile plants in the F2 population are chosen for gene mapping. Quantitative reverse-transcription real-time PCR assay and in situ hybridization	Oryza sativa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acyl-CoA + sn-glycerol 3-phosphate	-	Oryza sativa	CoA + 1-acyl-sn-glycerol 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
glycerol-3-phosphate acyltransferase 3	-	Oryza sativa
OsGPAT3	-	Oryza sativa

General Information

General Information	Comment	Organism
malfunction	loss of function of OsGPAT3 expression in tapetum and microspores disrupts tapetum development and metabolism, leading to defective anther cuticle and pollen exine formation. Compared with wild-type plants, the osgpat3 mutant displays smaller, pale yellow anthers with defective anther cuticle, degenerated pollen with defective exine, and abnormal tapetum development and degeneration. Anthers of the osgpat3 mutant have dramatic reductions of all aliphatic lipid contents. The defective cuticle and pollen phenotype coincide well with the down-regulation of sets of genes involved in lipid metabolism and regulation of anther development. The enzyme-deficient osgpat3 mutant exhibits normal vegetative development and inflorescence morphology, but has pale yellow to white and much smaller anthers compared with those of the wild-type and lacks mature pollen grains at the late stages of anther development, phenotype, detailed overview	Oryza sativa
physiological function	glycerol-3-phosphate acyltransferase 3 (OsGPAT3) is required for male fertility in rice. The endoplasmic reticulum-localized isozyme plays an indispensable role in anther development and pollen formation in rice. OsGPAT3 is essential for the timely differentiation and degradation of the tapetum	Oryza sativa