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Literature summary for 2.3.1.15 extracted from
- Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis (2017), Front. Plant Sci., 8, 1337 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene GPAT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa in Arabidopsis thaliana, quantitative real-time PCR expression analysis | Suaeda salsa |
| gene GPAT2, sequence comparisons and phylogenetic tree, quantitative real-time PCR enzyme expression analysis | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of a T-DNA insertion mutant strain of GPAT2, determination of chlorophyll content and fatty acids composition of phosphatidylglycerol (PG) in Arabidopsis thaliana GPAT6 mutant line, overview. In the seedling stage, chlorophyll content, the photochemical efficiency of PSII, PSI oxidoreductive activity (1I/Io), and the unsaturated fatty acid content of PG decrease less in overexpressing strains and more in mutant strains than that in wild-type under salt stress. Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis thaliana deficient in GPAT2. The overexpression of SsGPAT alleviates the photoinhibition of PSII and PSI under salt stress by improving the unsaturated fatty acid content of PG | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Suaeda salsa | 9507 | - |
| chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | synthesis of unsaturated fatty acid and double bond index (DBI) of phosphatidylglycerol of plants are inhibited by salt stress | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + sn-glycerol 3-phosphate | Suaeda salsa | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |  |
| acyl-CoA + sn-glycerol 3-phosphate | Arabidopsis thaliana | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + sn-glycerol 3-phosphate | Arabidopsis thaliana Col-0 | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9FZ22 | - |
- |
| Arabidopsis thaliana Col-0 | Q9FZ22 | - |
- |
| Suaeda salsa | - |
seeds collected during November 2013 in the saline inland of the Yellow River Delta (N37°25', E118°58') in Shandong province, China | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
| leaf | real-time PCR reveals that the mRNA accumulation of GPAT in Sueda salsa is induced by salt stress. The highest expression levels are observed when leaves are exposed to 300 mM NaCl treatment | Suaeda salsa | - |
| seedling | - |
Suaeda salsa | - |
| seedling | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + sn-glycerol 3-phosphate | - |
Suaeda salsa | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + sn-glycerol 3-phosphate | - |
Arabidopsis thaliana | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
| acyl-CoA + sn-glycerol 3-phosphate | - |
Arabidopsis thaliana Col-0 | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AT1G02390 | locus name | Arabidopsis thaliana |
| AtGPAT2 | - |
Arabidopsis thaliana |
| glycerol-3-phosphate acyltransferase | - |
Suaeda salsa |
| glycerol-3-phosphate acyltransferase | - |
Arabidopsis thaliana |
| GPAT | - |
Suaeda salsa |
| GPAT2 | - |
Arabidopsis thaliana |
| SsGPAT | - |
Suaeda salsa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Suaeda salsa | real-time PCR reveals that the mRNA accumulation of GPAT in Sueda salsa is induced by salt stress. The highest expression levels are observed when leaves are exposed to 300 mM NaCl treatment | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | GPAT2 mutant seeds show reduced germination rates and root lengths compared to wild-type in presence of 50-150 mM salt. Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis thaliana deficient in GPAT2. In the seedling stage, chlorophyll content, the photochemical efficiency of PSII, PSI oxidoreductive activity (1I/Io), and the unsaturated fatty acid content of PG decrease less in overexpressing strains and more in mutant strains than that in wild-type under salt stress. The overexpression of SsGPAT alleviates the photoinhibition of PSII and PSI under salt stress by improving the unsaturated fatty acid content of phosphatidylglycerol (PG) | Arabidopsis thaliana |
| malfunction | overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis thaliana deficient in GPAT (Arabidopsis thaliana mutants of gpat2 and gpat6) | Suaeda salsa |
| metabolism | glycerol-3-phosphate acyltransferase (GPAT) is the first acyl esterifying enzyme in phosphatidylglycerol (PG) synthesis process | Suaeda salsa |
| metabolism | glycerol-3-phosphate acyltransferase (GPAT) is the first acyl esterifying enzyme in phosphatidylglycerol (PG) synthesis process | Arabidopsis thaliana |
| physiological function | Suaeda salsa enzyme GPAT (SsGPAT) is involved in the plant's resistance to salt stress. Salt stress is known to inhibit photosynthesis through the process of photoinhibition. SsGPAT alleviates the photoinhibition of PSII and PSI under salt stress by improving the unsaturated fatty acid content of phosphatidylglycerol (PG). The enzyme transfers the acyl moiety from an acyl-coenzyme A (CoA) donor (or acyl-acyl carrier protein [ACP] in plastids) to the sn-1 position of a glycerol-3-phosphate (G3P) molecule, yielding 1-acylglycerol-3-phosphate (or lysophosphatidic acid, LPA) | Suaeda salsa |
| physiological function | the enzyme transfers the acyl moiety from an acyl-coenzyme A (CoA) donor (or acyl-acyl carrier protein [ACP] in plastids) to the sn-1 position of a glycerol-3-phosphate (G3P) molecule, yielding 1-acylglycerol-3-phosphate (or lysophosphatidic acid, LPA) | Arabidopsis thaliana |
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