Application | Comment | Organism |
---|---|---|
synthesis | potential of genetic manipulation of GPAT to increase the glycerolipid level in Lobosphaera incisa and other microalgae | Lobosphaera incisa |
Cloned (Comment) | Organism |
---|---|
gene LiGPAT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, detailed overview. Heterologous expression of wild-type mLiGPAT and mutant sdmLiGPAT R195H in the GPAT-deficient yeast mutant gat1DELTA. Recombinant expression of soluble His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS | Lobosphaera incisa |
Protein Variants | Comment | Organism |
---|---|---|
R195H | site-directed mutagenesis, the mutant cells show increased phospholipid levels | Lobosphaera incisa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | 63 residues at the N-terminus of the enzyme sequence are defined a chloroplast transit peptide | Lobosphaera incisa | 9507 | - |
additional information | the enzyme does not contain a transmembrane domain | Lobosphaera incisa | - |
- |
soluble | - |
Lobosphaera incisa | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Lobosphaera incisa | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | Lobosphaera incisa H4301 | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lobosphaera incisa | A0A0B5EHP8 | - |
- |
Lobosphaera incisa H4301 | A0A0B5EHP8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Lobosphaera incisa | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | - |
Lobosphaera incisa H4301 | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | LiGPAT tertiary structure modelling | Lobosphaera incisa |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-phosphate acyltransferase | - |
Lobosphaera incisa |
GPAT | - |
Lobosphaera incisa |
LiGPAT | - |
Lobosphaera incisa |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis of GPAT homologues | Lobosphaera incisa |
malfunction | lipid profile analyses of the transgenic yeasts validate the acylation function of LiGPAT and also indicate that mutation R195H leads to an increase in the phospholipid level in yeast, caused by the enlarged accessible surface of the phosphate group binding pocket when Arg195 is mutated to His | Lobosphaera incisa |
additional information | identification and quantification of lipid metabolites in wild-type and mutant strains | Lobosphaera incisa |