Application | Comment | Organism |
---|---|---|
medicine | major finding of the study is that overexpressing GPAT in liver causes hepatic steatosis and hepatic insulin resistance in the absence of obesity or high-fat feeding | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
hepatic overexpression of the enzyme with an adenoviral construct | Rattus norvegicus |
recombinant GPAT1-FLAG adenovirus and Ad-EGFP are constructed | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Rattus norvegicus | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | hepatic overexpression of glycerol-sn-3-phosphate acyltransferase 1 in rats causes insulin resistance | Rattus norvegicus | - |
muscle | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0003 | - |
activity in rat liver membranes after inhibiting endoplasmic reticulum GPAT with N-ethylmaleimide, rats are treated with Ad-EGFP | Rattus norvegicus |
0.00083 | - |
activity in rat liver membranes after inhibiting endoplasmic reticulum GPAT with N-ethylmaleimide, rats are treated with Ad-GPAT1 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Rattus norvegicus | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
fatty acyl-CoA + glycerol-3-phosphate | - |
Rattus norvegicus | CoA + lysophosphatidic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acyl-CoA:glycerol-3-phosphate acyltransferase 1 | - |
Rattus norvegicus |
Ad-GPAT1 | adenoviral GPAT1 construct | Rattus norvegicus |
glycerol-sn-3-phosphate acyltransferase 1 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
activity assay at room temperature | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay | Rattus norvegicus |